pPE3
(Plasmid
#237896)
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Purpose(Empty Backbone) Lentiviral backbone for expressing multiple pegRNAs/sgRNAs in pPapi expression cassette
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Depositing Lab
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Depositing OrganizationMartin-Luther-Universitaet Halle-Wittenberg, Faculty of Medicine
Located in Germany -
Sequence Information
Ordering
This material is available to academics and nonprofits only.
| Item | Catalog # | Description | Quantity | Price (USD) | |
|---|---|---|---|---|---|
| Plasmid | 237896 | Standard format: Plasmid sent in bacteria as agar stab | 1 | $94 | |
Backbone
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Vector backbonesgLenti #105996
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Modifications to backboneBsmBI restriction site was removed. The pPapi expression cassette (u6-stuffer-H1) was amplified from vector pPapi (Addgene plasmid #96921) and cloned into sgLenti (Addgene plasmid #105996).
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Vector typeMammalian Expression, Lentiviral, CRISPR
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Selectable markersPuromycin ; mCherry
Growth in Bacteria
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Bacterial Resistance(s)Ampicillin, 100 μg/mL
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Growth Temperature37°C
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Growth Strain(s)NEB Stable
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Copy numberUnknown
Cloning Information
- Cloning method Gibson Cloning
Resource Information
Terms and Licenses
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Academic/Nonprofit Terms
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Industry Terms
- Not Available to Industry
Trademarks:
- Zeocin® is an InvivoGen trademark.
How to cite this plasmid
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These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.
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For your Materials & Methods section:
pPE3 was a gift from Michael Boettcher (Addgene plasmid # 237896 ; http://n2t.net/addgene:237896 ; RRID:Addgene_237896)
Map uploaded by the depositor.
