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pME463
(Plasmid #238103)

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This material is available to academics and nonprofits only.
Item Catalog # Description Quantity Price (USD)
Plasmid 238103 Standard format: Plasmid sent in bacteria as agar stab 1 $94
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DNA 238103-D50 50 μg of DNA in Tris buffer $495
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DNA 238103-D100 100 μg of DNA in Tris buffer $585
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Backbone

  • Vector backbone
    pCMV
  • Total vector size (bp) 9024
  • Vector type
    Mammalian Expression, CRISPR

Growth in Bacteria

  • Bacterial Resistance(s)
    Ampicillin, 100 μg/mL
  • Growth Temperature
    37°C
  • Growth Strain(s)
    NEB Stable
  • Copy number
    High Copy

Gene/Insert

  • Gene/Insert name
    TadA*7.10(Y123H/N157K)-m_SpRYnCas9(D10A)
  • Alt name
    ABE7.10-HK SpRY-nCas9
  • Species
    H. sapiens (human), Synthetic
  • Insert Size (bp)
    5625
  • Mutation
    TadA modifications include Y123H, N157K; SpRY-nCas9 modifications include D10A (nick) and A61R, L111R, N1317R, A1322R, and R1333P (SpRY)
  • Tags / Fusion Proteins
    • SV40 bpNLS-P2A-mCherry (C terminal on insert)
    • SV40 bpNLS (N terminal on insert)

Resource Information

Terms and Licenses

  • Academic/Nonprofit Terms
  • Industry Terms
    • Not Available to Industry

Trademarks:

  • Zeocin® is an InvivoGen trademark.

Purpose

Ready-to-use, high-purity DNA prep. DNA aliquots are suitable for use in mammalian cell transfections and other molecular biology applications.

Delivery

  • Amount 50 μg
  • Concentration 1 μg/μL
  • Pricing $495 USD
  • Storage 4 ℃ (short-term) or -20 ℃ (long-term)

Terms and Licenses

  • Academic/Nonprofit Terms
  • Industry Terms
    • Not Available to Industry

Quality Control

Every DNA prep is verified by next-generation sequencing, confirming plasmid identity, sequence integrity, and absence of DNA contaminants. Concentration is confirmed to be within ±2.5% of the stated concentration.

Our production process has been validated to consistently yield endotoxin levels below 1.0 EU/μg. Individual lots are not tested for endotoxin.

Purpose

Ready-to-use, high-purity DNA prep. DNA aliquots are suitable for use in mammalian cell transfections and other molecular biology applications.

Delivery

  • Amount 100 μg
  • Concentration 1 μg/μL
  • Pricing $585 USD
  • Storage 4 ℃ (short-term) or -20 ℃ (long-term)

Terms and Licenses

  • Academic/Nonprofit Terms
  • Industry Terms
    • Not Available to Industry

Quality Control

Every DNA prep is verified by next-generation sequencing, confirming plasmid identity, sequence integrity, and absence of DNA contaminants. Concentration is confirmed to be within ±2.5% of the stated concentration.

Our production process has been validated to consistently yield endotoxin levels below 1.0 EU/μg. Individual lots are not tested for endotoxin.

How to cite this plasmid ( Back to top)

These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.

  • For your Materials & Methods section:

    pME463 was a gift from Alexis Komor (Addgene plasmid # 238103 ; http://n2t.net/addgene:238103 ; RRID:Addgene_238103)

  • For your References section:

    Precise, minimally evolved adenine base editors generated through mutation reversion analysis. Evanoff M, Korpal S, Krill ZD, Cowan QT, Komor AC. Nat Biotechnol. 2026 Mar 18. doi: 10.1038/s41587-026-03045-z. 10.1038/s41587-026-03045-z PubMed 41851306
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