pCMV-dCas13-NES-eNAT10
(Plasmid
#238299)
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PurposeExpresses cytoplasmic version of the programmable RNA acetylation system.
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Depositing Lab
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Depositing OrganizationKorea Advanced Institute of Science and Technology
Located in the Republic of Korea -
Sequence Information
Ordering
| Item | Catalog # | Description | Quantity | Price (USD) | |
|---|---|---|---|---|---|
| Plasmid | 238299 | Standard format: Plasmid sent in bacteria as agar stab | 1 | $94 | |
| DNA | 238299-D50 | 50 μg of DNA in Tris buffer | $495 | ||
| DNA | 238299-D100 | 100 μg of DNA in Tris buffer | $585 | ||
Backbone
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Vector backboneEBFP2-N1
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Backbone manufacturerClontech
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Vector typeMammalian Expression, CRISPR
Growth in Bacteria
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Bacterial Resistance(s)Kanamycin, 50 μg/mL
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Growth Temperature37°C
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Growth Strain(s)DH5alpha
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Copy numberHigh Copy
Gene/Insert
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Gene/Insert namedPspCas13b fused to eNAT10
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SpeciesH. sapiens (human); Prevotella sp. P5-125
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MutationH133A for catalytically inactive mutant, deletion of aa984-1090 in PspCas13b; deletion of aa802-1025 from hNAT10
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Entrez GeneNAT10 (a.k.a. ALP, Kre33, NET43)
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Tag
/ Fusion Protein
- HIV NES (C terminal of dPspCas13b)
Resource Information
Terms and Licenses
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Academic/Nonprofit Terms
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Industry Terms
- Not Available to Industry
Trademarks:
- Zeocin® is an InvivoGen trademark.
DNA (Catalog # 238299-D50)
Purpose
Ready-to-use, high-purity DNA prep. DNA aliquots are suitable for use in mammalian cell transfections and other molecular biology applications.
Delivery
- Amount 50 μg
- Concentration 1 μg/μL
- Pricing $495 USD
- Storage 4 ℃ (short-term) or -20 ℃ (long-term)
Terms and Licenses
-
Academic/Nonprofit Terms
-
Industry Terms
- Not Available to Industry
Quality Control
Every DNA prep is verified by next-generation sequencing, confirming plasmid identity, sequence integrity, and absence of DNA contaminants. Concentration is confirmed to be within ±2.5% of the stated concentration.
Our production process has been validated to consistently yield endotoxin levels below 1.0 EU/μg. Individual lots are not tested for endotoxin.
DNA (Catalog # 238299-D100)
Purpose
Ready-to-use, high-purity DNA prep. DNA aliquots are suitable for use in mammalian cell transfections and other molecular biology applications.
Delivery
- Amount 100 μg
- Concentration 1 μg/μL
- Pricing $585 USD
- Storage 4 ℃ (short-term) or -20 ℃ (long-term)
Terms and Licenses
-
Academic/Nonprofit Terms
-
Industry Terms
- Not Available to Industry
Quality Control
Every DNA prep is verified by next-generation sequencing, confirming plasmid identity, sequence integrity, and absence of DNA contaminants. Concentration is confirmed to be within ±2.5% of the stated concentration.
Our production process has been validated to consistently yield endotoxin levels below 1.0 EU/μg. Individual lots are not tested for endotoxin.
These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.
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For your Materials & Methods section:
pCMV-dCas13-NES-eNAT10 was a gift from Won Do Heo (Addgene plasmid # 238299 ; http://n2t.net/addgene:238299 ; RRID:Addgene_238299) -
For your References section:
Programmable RNA acetylation with CRISPR-Cas13. Yu J, Jin J, Kwon E, Jang H, Choi SK, Kim D, Kim C, Son S, Yoon KJ, Heo WD. Nat Chem Biol. 2025 Jun 2. doi: 10.1038/s41589-025-01922-3. 10.1038/s41589-025-01922-3 PubMed 40456962
Map uploaded by the depositor.
