pcDNA5 FRT TO LRRK2_turboID-GA5
(Plasmid
#238506)
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PurposeExpresses turboID-LRRK2 without 3xFLAG in mammalian cells
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Depositing Lab
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Depositing OrganizationUniversitaet Konstanz
Located in Germany -
Publication
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Sequence Information
Ordering
| Item | Catalog # | Description | Quantity | Price (USD) | |
|---|---|---|---|---|---|
| Plasmid | 238506 | Standard format: Plasmid sent in bacteria as agar stab | 1 | $94 | |
Backbone
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Vector backbonepcDNA5
- Total vector size (bp) 13668
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Vector typeMammalian Expression
Growth in Bacteria
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Bacterial Resistance(s)Ampicillin, 100 μg/mL
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Growth Temperature37°C
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Growth Strain(s)XL10 Gold
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Growth instructionsDH5alpha not recommended due to low yield
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Copy numberUnknown
Gene/Insert
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Gene/Insert nameLeucine-rich repeat serine/threonine-protein kinase 2
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Alt nameLRRK2
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SpeciesH. sapiens (human)
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Insert Size (bp)7584
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Entrez GeneLRRK2 (a.k.a. AURA17, DARDARIN, PARK8, RIPK7, ROCO2)
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Tag
/ Fusion Protein
- turboID (N terminal on insert)
Terms and Licenses
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Academic/Nonprofit Terms
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Industry Terms
- Not Available to Industry
Trademarks:
- Zeocin® is an InvivoGen trademark.
These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.
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For your Materials & Methods section:
pcDNA5 FRT TO LRRK2_turboID-GA5 was a gift from Florian Stengel (Addgene plasmid # 238506 ; http://n2t.net/addgene:238506 ; RRID:Addgene_238506) -
For your References section:
Probing the proteome-wide impact of inhibitors of Leucine-rich Repeat Kinases 1 and 2 on protein-protein interactions and phosphorylation. Jansen J, Wendel M, Raig ND, Kraus T, Surridge KJ, Mahesula S, Richter-Müller N, Mathea S, Reck-Peterson SL, Knapp S, Stengel F. bioRxiv 2025.09.03.674114 10.1101/2025.09.03.674114