MCP-Rep-X-RT∆R
(Plasmid
#239384)
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PurposeFor circular RNA-mediated inverse prime editor using MCP-Rep-X-M MLV-RT∆RNase H in HEK294T cells
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Depositing Lab
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Depositing OrganizationInstitute of Genetics and Developmental Biology, Chinese Academy of Sciences
Located in China -
Sequence Information
Ordering
| Item | Catalog # | Description | Quantity | Price (USD) | |
|---|---|---|---|---|---|
| Plasmid | 239384 | Standard format: Plasmid sent in bacteria as agar stab | 1 | $94 | |
| DNA | 239384-D50 | 50 μg of DNA in Tris buffer | $495 | ||
| DNA | 239384-D100 | 100 μg of DNA in Tris buffer | $585 | ||
Backbone
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Vector backboneMCP-M-MLV RT∆RNase H (Addgene #213749)
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Backbone manufacturerCaixia Gao
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Vector typeMammalian Expression, CRISPR
Growth in Bacteria
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Bacterial Resistance(s)Ampicillin, 100 μg/mL
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Growth Temperature37°C
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Growth Strain(s)DH5alpha
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Copy numberHigh Copy
Gene/Insert
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Gene/Insert nameMCP, Rep-X, M-MLV-RT(∆RNase H)
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SpeciesMS2 phage, E. coli, MMLV
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Mutation∆RNase H in M-MLV RT
- Promoter CMV
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Tags
/ Fusion Proteins
- BPNLS (N terminal on insert)
- BPNLS (C terminal on insert)
Cloning Information
- Cloning method Gibson Cloning
- 5′ sequencing primer n/a
- (Common Sequencing Primers)
Resource Information
Terms and Licenses
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Academic/Nonprofit Terms
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Industry Terms
- Not Available to Industry
Trademarks:
- Zeocin® is an InvivoGen trademark.
DNA (Catalog # 239384-D50)
Purpose
Ready-to-use, high-purity DNA prep. DNA aliquots are suitable for use in mammalian cell transfections and other molecular biology applications.
Delivery
- Amount 50 μg
- Concentration 1 μg/μL
- Pricing $495 USD
- Storage 4 ℃ (short-term) or -20 ℃ (long-term)
Terms and Licenses
-
Academic/Nonprofit Terms
-
Industry Terms
- Not Available to Industry
Quality Control
Every DNA prep is verified by next-generation sequencing, confirming plasmid identity, sequence integrity, and absence of DNA contaminants. Concentration is confirmed to be within ±2.5% of the stated concentration.
Our production process has been validated to consistently yield endotoxin levels below 1.0 EU/μg. Individual lots are not tested for endotoxin.
DNA (Catalog # 239384-D100)
Purpose
Ready-to-use, high-purity DNA prep. DNA aliquots are suitable for use in mammalian cell transfections and other molecular biology applications.
Delivery
- Amount 100 μg
- Concentration 1 μg/μL
- Pricing $585 USD
- Storage 4 ℃ (short-term) or -20 ℃ (long-term)
Terms and Licenses
-
Academic/Nonprofit Terms
-
Industry Terms
- Not Available to Industry
Quality Control
Every DNA prep is verified by next-generation sequencing, confirming plasmid identity, sequence integrity, and absence of DNA contaminants. Concentration is confirmed to be within ±2.5% of the stated concentration.
Our production process has been validated to consistently yield endotoxin levels below 1.0 EU/μg. Individual lots are not tested for endotoxin.
These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.
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For your Materials & Methods section:
MCP-Rep-X-RT∆R was a gift from Caixia Gao (Addgene plasmid # 239384 ; http://n2t.net/addgene:239384 ; RRID:Addgene_239384) -
For your References section:
Circular RNA-mediated inverse prime editing in human cells. Liang R, Wang S, Cai Y, Li Z, Li KM, Wei J, Sun C, Zhu H, Chen K, Gao C. Nat Commun. 2025 May 31;16(1):5057. doi: 10.1038/s41467-025-59120-7. 10.1038/s41467-025-59120-7 PubMed 40447589
