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pKS1616
(Plasmid #239547)

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  • Purpose
    Express GAL-inducible G8H cytochrome P450 and associated reductase as membrane protein cargoes and inactive Pex22 peroxisomal chauffeur; chromosomal integration by CRISPR
  • Depositing Lab
    John Dueber
  • Depositing Organization
    University of California, Berkeley
    Located in United States of America
  • Publication
    Dueber Lab plasmids 2025 (unpublished) ( How to cite )
  • Sequence Information

Ordering

This material is available to academics and nonprofits only.
Item Catalog # Description Quantity Price (USD)
Plasmid 239547 Standard format: Plasmid sent in bacteria as agar stab 1 $94
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Backbone

  • Vector backbone
    PSG748
  • Backbone manufacturer
    Internal
  • Vector type
    Yeast Expression

Growth in Bacteria

  • Bacterial Resistance(s)
    Kanamycin, 50 μg/mL
  • Growth Temperature
    37°C
  • Growth Strain(s)
    DH5alpha
  • Copy number
    High Copy

Gene/Insert

  • Gene/Insert name
    CrG8H-2xCrk_SH3
  • Species
    Catharanthus roseus

Cloning Information

  • Cloning method Golden Gate

Resource Information

Terms and Licenses

  • Academic/Nonprofit Terms
  • Industry Terms
    • Not Available to Industry

Trademarks:

  • Zeocin® is an InvivoGen trademark.

Depositor Comments

Additional inserts: 1) CrCPR-2xCrk_SH3; 2) ScPex22(1-36)-mRuby2-SH3pep_dead

How to cite this plasmid ( Back to top)

These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.

  • For your Materials & Methods section:

    pKS1616 was a gift from John Dueber (Addgene plasmid # 239547 ; http://n2t.net/addgene:239547 ; RRID:Addgene_239547)
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