pcDNA3 Ub-UBB+1
(Plasmid #23967)

• Depositing Lab: Nico Dantuma
• Publication: Lindsten et al J Cell Biol. 2002 Apr 29. 157(3):417-27.

Backbone

• Vector backbone: pcDNA3
• Vector type: Mammalian Expression

Growth in Bacteria

• Bacterial Resistance(s): Ampicillin, 100 μg/mL
• Growth Temperature: 37°C
• Growth Strain(s): DH5alpha
• Copy number: High Copy

Gene/Insert

• Gene/Insert name: Ubiquitin B +1 transcript
• Alt name: Ubb+1
• Alt name: Ub-Ubb+1
• Species: H. sapiens (human)
• Entrez Gene: UBB (a.k.a. HEL-S-50)

Cloning Information

• Cloning method: Restriction Enzyme
• 5′ cloning site: - (unknown if destroyed)
• 3′ cloning site: - (unknown if destroyed)
• 5′ sequencing primer: n/a

Terms and Licenses

• Academic/Nonprofit Terms:
  • UBMTA
• Industry Terms:
  • Not Available to Industry

Depositor Comments

There is additional sequence in front of the Ubiquitin ATG that does not affect plasmid function.