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pBAD YuzuFP
(Plasmid #240149)

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This material is available to academics and nonprofits only.
Item Catalog # Description Quantity Price (USD)
Plasmid 240149 Standard format: Plasmid sent in bacteria as agar stab 1 $94
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Backbone

  • Vector backbone
    pBAD
  • Vector type
    Bacterial Expression

Growth in Bacteria

  • Bacterial Resistance(s)
    Ampicillin, 100 μg/mL
  • Growth Temperature
    37°C
  • Growth Strain(s)
    DH5alpha
  • Copy number
    Unknown

Gene/Insert

  • Gene/Insert name
    YuzuFP
  • Alt name
    sfGFP H148S
  • Species
    Aequorea victoria
  • Insert Size (bp)
    717
  • Mutation
    H148S
  • GenBank ID
    PV666611
  • Promoter Arabinose
  • Tag / Fusion Protein
    • 6xHis tag (C terminal on backbone)

Cloning Information

Resource Information

  • A portion of this plasmid was derived from a plasmid made by
    based on sfGFP from Michael Davidson, Addgene Plasmid #54519

Terms and Licenses

  • Academic/Nonprofit Terms
  • Industry Terms
    • Not Available to Industry

Trademarks:

  • Zeocin® is an InvivoGen trademark.

Depositor Comments

Please visit https://doi.org/10.1101/2025.01.08.631926 for bioRxiv preprint.

How to cite this plasmid ( Back to top)

These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.

  • For your Materials & Methods section:

    pBAD YuzuFP was a gift from Dafydd Jones (Addgene plasmid # 240149 ; http://n2t.net/addgene:240149 ; RRID:Addgene_240149)

  • For your References section:

    Molecular dynamics guided identification of a brighter variant of superfolder Green Fluorescent Protein with increased photobleaching resistance. Ahmed RD, Jamieson WD, Vitsupakorn D, Zitti A, Pawson KA, Castell OK, Watson PD, Jones DD. Commun Chem. 2025 Jun 5;8(1):174. doi: 10.1038/s42004-025-01573-4. 10.1038/s42004-025-01573-4 PubMed 40473962
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