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pENTR_sfGFP-TurboID
(Plasmid #240226)

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This material is available to academics and nonprofits only.
Item Catalog # Description Quantity Price (USD)
Plasmid 240226 Standard format: Plasmid sent in bacteria as agar stab 1 $94
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DNA 240226-D50 50 μg of DNA in Tris buffer $495
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DNA 240226-D100 100 μg of DNA in Tris buffer $585
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Backbone

  • Vector backbone
    pEntr
  • Backbone manufacturer
    Thermo Fisher
  • Vector type
    Gateway shuttle vector

Growth in Bacteria

  • Bacterial Resistance(s)
    Kanamycin, 50 μg/mL
  • Growth Temperature
    37°C
  • Growth Strain(s)
    DH5alpha
  • Copy number
    Unknown

Gene/Insert

  • Gene/Insert name
    sfGFP-TurboID
  • Species
    Synthetic

Cloning Information

  • Cloning method Gibson Cloning

Terms and Licenses

  • Academic/Nonprofit Terms
  • Industry Terms
    • Not Available to Industry

Trademarks:

  • Zeocin® is an InvivoGen trademark.

Depositor Comments

Please visit https://doi.org/10.1101/2025.07.09.659702 for bioRxiv preprint.

Purpose

Ready-to-use, high-purity DNA prep. DNA aliquots are suitable for use in mammalian cell transfections and other molecular biology applications.

Delivery

  • Amount 50 μg
  • Concentration 1 μg/μL
  • Pricing $495 USD
  • Storage 4 ℃ (short-term) or -20 ℃ (long-term)

Terms and Licenses

  • Academic/Nonprofit Terms
  • Industry Terms
    • Not Available to Industry

Quality Control

Every DNA prep is verified by next-generation sequencing, confirming plasmid identity, sequence integrity, and absence of DNA contaminants. Concentration is confirmed to be within ±2.5% of the stated concentration.

Our production process has been validated to consistently yield endotoxin levels below 1.0 EU/μg. Individual lots are not tested for endotoxin.

Purpose

Ready-to-use, high-purity DNA prep. DNA aliquots are suitable for use in mammalian cell transfections and other molecular biology applications.

Delivery

  • Amount 100 μg
  • Concentration 1 μg/μL
  • Pricing $585 USD
  • Storage 4 ℃ (short-term) or -20 ℃ (long-term)

Terms and Licenses

  • Academic/Nonprofit Terms
  • Industry Terms
    • Not Available to Industry

Quality Control

Every DNA prep is verified by next-generation sequencing, confirming plasmid identity, sequence integrity, and absence of DNA contaminants. Concentration is confirmed to be within ±2.5% of the stated concentration.

Our production process has been validated to consistently yield endotoxin levels below 1.0 EU/μg. Individual lots are not tested for endotoxin.

How to cite this plasmid ( Back to top)

These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.

  • For your Materials & Methods section:

    pENTR_sfGFP-TurboID was a gift from Justin Bosch & Norbert Perrimon (Addgene plasmid # 240226 ; http://n2t.net/addgene:240226 ; RRID:Addgene_240226)

  • For your References section:

    Multi-omic mapping of Drosophila protein secretomes reveals tissue-specific origins and inter-organ trafficking. Bosch JA, Beltran PMJ, Cavers C, LaGraff JT, Melanson R, Singh A, Chen W, Hu Y, Tattikota SG, Liu Y, Hashmi Y, Asara JM, Branon T, Ting AY, Carr SA, Perrimon N. Nat Commun. 2026 Apr 20. doi: 10.1038/s41467-026-71763-8. 10.1038/s41467-026-71763-8 PubMed 42009678
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