pRK793-Strep
(Plasmid #240334)

• Purpose: Express StreptagII-TEV
• Depositing Lab: Simone Morra
• Publication: Alogaidi et al J Am Chem Soc. 2026 Jul 10. doi: 10.1021/jacs.6c05865.

Backbone

• Vector backbone: pRK793
• Backbone manufacturer: David Waugh (Addgene plasmid # 8827)
• Total vector size (bp): 7411
• Modifications to backbone: Contains StrepTagII sequence instead of HisTag
• Vector type: Bacterial Expression

Growth in Bacteria

• Bacterial Resistance(s): Ampicillin, 100 μg/mL
• Growth Temperature: 37°C
• Growth Strain(s): DH5alpha
• Growth instructions: Use Rosetta2(DE3) for protein expression and purification.
• Copy number: Low Copy

Gene/Insert

• Gene/Insert name: TEV protease, S219V mutant with StrepTagII
• Alt name: tobacco etch virus protease
• Mutation: S219V mutation (improves stability)
• Tags / Fusion Proteins:
  • StrepTagII (N terminal on insert)
  • polyarginine (C terminal on insert)
  • MBP (gets cleaved post translation) (N terminal on insert)

Resource Information

• A portion of this plasmid was derived from a plasmid made by: The original plasmid was 8827 from Addgene. We replaced the affinity tag (now has StrepTagII rather than HisTag).

Terms and Licenses

• Academic/Nonprofit Terms:
  • UBMTA
• Industry Terms:
  • Not Available to Industry