pET15Sumo2_Dengue-NS2B-NS3_GTM_001
(Plasmid #240378)

• Purpose: Expresses Dengue NS2b-NS3 protease in E. coli
• Depositing Lab: Gaetano Montelione
• Publication: A PA CLAN PROTEASE CROSS-INHIBITION SCREEN (unpublished)

Backbone

• Vector backbone: pET15SUMO2
• Backbone manufacturer: Novagen/NESG
• Backbone size w/o insert (bp): 5681
• Total vector size (bp): 6397
• Modifications to backbone: An N-terminal 6xHis tag fused to the Smt3 gene (Sumo) of Saccharomyces cerevisiae as a purification/solubility enhancement cassette. BsaI sites are included in the cloning polylinker for scarless cloning and the production of a native, mature protein after cleavage from the Sumo fusion by Sumo protease (Ulp1).
• Vector type: Bacterial Expression

Growth in Bacteria

• Bacterial Resistance(s): Ampicillin, 100 μg/mL
• Growth Temperature: 37°C
• Growth Strain(s): DH5alpha
• Growth instructions: Grow in BL21 DE3 for expression
• Copy number: Low Copy

Gene/Insert

• Gene/Insert name: DNV_NS2B-NS3 protease
• Species: Dengue virus
• Insert Size (bp): 716
• Mutation: NS2B-NS3 peptides fused by Gly-Ser linker
• GenBank ID:
• Promoter: T7 lac
• Tag / Fusion Protein:
  • 6xHis, Sumo (N terminal on backbone)

Cloning Information

• Cloning method: Unknown
• 5′ sequencing primer: T7 forward

Resource Information

• A portion of this plasmid was derived from a plasmid made by: This construct was produced by Genscript USA

Terms and Licenses

• Academic/Nonprofit Terms:
  • UBMTA
• Industry Terms:
  • Not Available to Industry

Depositor Comments

Related to PDB: 2M9P