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pET15Sumo2-SARS-CoV-3CLpro_GTM_001
(Plasmid #240380)

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This material is available to academics and nonprofits only. Currently unavailable outside the U.S.
Item Catalog # Description Quantity Price (USD)
Plasmid 240380 Standard format: Plasmid sent in bacteria as agar stab 1 $94
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Backbone

  • Vector backbone
    pET15SUMO2
  • Backbone manufacturer
    Novagen/NESG
  • Backbone size w/o insert (bp) 5681
  • Total vector size (bp) 6604
  • Modifications to backbone
    An N-terminal 6xHis tag fused to the Smt3 gene (Sumo) of Saccharomyces cerevisiae as a purification/solubility enhancement cassette. BsaI sites are included in the cloning polylinker for scarless cloning and the production of a native, mature protein after cleavage from the Sumo fusion by Sumo protease (Ulp1).
  • Vector type
    Bacterial Expression

Growth in Bacteria

  • Bacterial Resistance(s)
    Ampicillin, 100 μg/mL
  • Growth Temperature
    37°C
  • Growth Strain(s)
    DH5alpha
  • Growth instructions
    Grow in BL21 DE3 cells for expression
  • Copy number
    Low Copy

Gene/Insert

  • Gene/Insert name
    3CL protease
  • Species
    SARS coronavirus
  • Insert Size (bp)
    923
  • GenBank ID
  • Entrez Gene
    ORF1ab (a.k.a. sarsp1)
  • Promoter T7 lac
  • Tag / Fusion Protein
    • 6xHis, Sumo (N terminal on backbone)

Cloning Information

Resource Information

  • A portion of this plasmid was derived from a plasmid made by
    Construct purchased from Genscript USA

Terms and Licenses

  • Academic/Nonprofit Terms
  • Industry Terms
    • Not Available to Industry

Trademarks:

  • Zeocin® is an InvivoGen trademark.

Depositor Comments

Related to PDB: 4YLU

How to cite this plasmid ( Back to top)

These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.

  • For your Materials & Methods section:

    pET15Sumo2-SARS-CoV-3CLpro_GTM_001 was a gift from Gaetano Montelione (Addgene plasmid # 240380 ; http://n2t.net/addgene:240380 ; RRID:Addgene_240380)
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