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pGTM-Sumo2-WN-NS2-NS3Sh
(Plasmid #240392)

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This material is available to academics and nonprofits only.
Item Catalog # Description Quantity Price (USD)
Plasmid 240392 Standard format: Plasmid sent in bacteria as agar stab 1 $94
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Backbone

  • Vector backbone
    pET15SUMO2
  • Backbone manufacturer
    Novagen/NESG
  • Backbone size w/o insert (bp) 5681
  • Total vector size (bp) 6361
  • Vector type
    Bacterial Expression

Growth in Bacteria

  • Bacterial Resistance(s)
    Ampicillin, 100 μg/mL
  • Growth Temperature
    37°C
  • Growth Strain(s)
    DH5alpha
  • Growth instructions
    Grow in BL21 DE3 cells for expression
  • Copy number
    Low Copy

Gene/Insert

  • Gene/Insert name
    NS2B-NS3 protease
  • Species
    West Nile Virus
  • Insert Size (bp)
    680
  • Mutation
    NS2B-NS3 peptides fused by Gly-Ser linker
  • GenBank ID
    AFP50427.1
  • Promoter T7 lac
  • Tags / Fusion Proteins
    • 6xHis, SUMO (N terminal on backbone)
    • 6X-His tag - Sumo (N terminal on backbone)

Cloning Information

Resource Information

  • A portion of this plasmid was derived from a plasmid made by
    Construct purchased from Genscript USA

Terms and Licenses

  • Academic/Nonprofit Terms
  • Industry Terms
    • Not Available to Industry

Trademarks:

  • Zeocin® is an InvivoGen trademark.
How to cite this plasmid ( Back to top)

These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.

  • For your Materials & Methods section:

    pGTM-Sumo2-WN-NS2-NS3Sh was a gift from Gaetano Montelione (Addgene plasmid # 240392 ; http://n2t.net/addgene:240392 ; RRID:Addgene_240392)
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