pBluescript II NLS-MC-EGFP-tdTomato (pBET2)
(Plasmid
#240411)
-
PurposeThis plasmid facilitates the assessment of MMR proficiency in human living cells
-
Depositing Lab
-
Depositing OrganizationFukuoka University
Located in Japan -
Sequence Information
Ordering
| Item | Catalog # | Description | Quantity | Price (USD) | |
|---|---|---|---|---|---|
| Plasmid | 240411 | Standard format: Plasmid sent in bacteria as agar stab | 1 | $94 | |
| DNA | 240411-D50 | 50 μg of DNA in Tris buffer | $495 | ||
| DNA | 240411-D100 | 100 μg of DNA in Tris buffer | $585 | ||
Backbone
-
Vector backbonepBluescript II
- Total vector size (bp) 6712
-
Vector typeMammalian Expression
Growth in Bacteria
-
Bacterial Resistance(s)Ampicillin, 100 μg/mL
-
Growth Temperature37°C
-
Growth Strain(s)DH5alpha
-
Copy numberHigh Copy
Gene/Insert
-
Gene/Insert nameNLS-EGFP fusion protein
-
SpeciesSynthetic
-
GenBank IDLC663823.1
- Promoter CMV
Cloning Information
- Cloning method Other
- 5′ sequencing primer M13 fwd
- (Common Sequencing Primers)
Terms and Licenses
-
Academic/Nonprofit Terms
-
Industry Terms
- Not Available to Industry
Trademarks:
- Zeocin® is an InvivoGen trademark.
DNA (Catalog # 240411-D50)
Purpose
Ready-to-use, high-purity DNA prep. DNA aliquots are suitable for use in mammalian cell transfections and other molecular biology applications.
Delivery
- Amount 50 μg
- Concentration 1 μg/μL
- Pricing $495 USD
- Storage 4 ℃ (short-term) or -20 ℃ (long-term)
Terms and Licenses
-
Academic/Nonprofit Terms
-
Industry Terms
- Not Available to Industry
Quality Control
Every DNA prep is verified by next-generation sequencing, confirming plasmid identity, sequence integrity, and absence of DNA contaminants. Concentration is confirmed to be within ±2.5% of the stated concentration.
Our production process has been validated to consistently yield endotoxin levels below 1.0 EU/μg. Individual lots are not tested for endotoxin.
DNA (Catalog # 240411-D100)
Purpose
Ready-to-use, high-purity DNA prep. DNA aliquots are suitable for use in mammalian cell transfections and other molecular biology applications.
Delivery
- Amount 100 μg
- Concentration 1 μg/μL
- Pricing $585 USD
- Storage 4 ℃ (short-term) or -20 ℃ (long-term)
Terms and Licenses
-
Academic/Nonprofit Terms
-
Industry Terms
- Not Available to Industry
Quality Control
Every DNA prep is verified by next-generation sequencing, confirming plasmid identity, sequence integrity, and absence of DNA contaminants. Concentration is confirmed to be within ±2.5% of the stated concentration.
Our production process has been validated to consistently yield endotoxin levels below 1.0 EU/μg. Individual lots are not tested for endotoxin.
These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.
-
For your Materials & Methods section:
pBluescript II NLS-MC-EGFP-tdTomato (pBET2) was a gift from Isao Kuraoka (Addgene plasmid # 240411 ; http://n2t.net/addgene:240411 ; RRID:Addgene_240411) -
For your References section:
Novel plasmids for the fluorescence-based evaluation of DNA mismatch repair in human cells. Takedachi A, Matsuishi E, Mizusaki S, Nagasawa T, Fujikane R, Hidaka M, Iwai S, Kuraoka I. Mutat Res. 2022 Jan-Jun;824:111779. doi: 10.1016/j.mrfmmm.2022.111779. Epub 2022 Apr 9. 10.1016/j.mrfmmm.2022.111779 PubMed 35472567
