HA-KIF5A-FRB
(Plasmid
#240425)
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Purpose+end oriented kinesin for rapalog induced pulling experiments.
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Depositing Lab
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Depositing OrganizationUtrecht University
Located in the Netherlands -
Sequence Information
Ordering
| Item | Catalog # | Description | Quantity | Price (USD) | |
|---|---|---|---|---|---|
| Plasmid | 240425 | Standard format: Plasmid sent in bacteria as agar stab | 1 | $94 | |
| DNA | 240425-D50 | 50 μg of DNA in Tris buffer | $495 | ||
| DNA | 240425-D100 | 100 μg of DNA in Tris buffer | $585 | ||
Backbone
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Vector backbonepGFP-C2
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Vector typeMammalian Expression
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Selectable markersNeomycin (select with G418)
Growth in Bacteria
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Bacterial Resistance(s)Kanamycin, 50 μg/mL
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Growth Temperature37°C
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Growth Strain(s)DH5alpha
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Copy numberUnknown
Gene/Insert
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Gene/Insert nameKIF5A
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SpeciesM. musculus (mouse)
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Entrez GeneKif5a (a.k.a. D10Bwg0738e, Khc, Kif5, Kns, mKIAA4086)
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Tags
/ Fusion Proteins
- HA (N terminal on insert)
- FRB (C terminal on insert)
Resource Information
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A portion of this plasmid was derived from a plasmid made byGinny Farias, Utrecht University, The Netherlands
Terms and Licenses
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Academic/Nonprofit Terms
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Industry Terms
- Not Available to Industry
Trademarks:
- Zeocin® is an InvivoGen trademark.
Depositor Comments
Please visit https://doi.org/10.1101/2024.11.21.624725 for bioRxiv preprint.
DNA (Catalog # 240425-D50)
Purpose
Ready-to-use, high-purity DNA prep. DNA aliquots are suitable for use in mammalian cell transfections and other molecular biology applications.
Delivery
- Amount 50 μg
- Concentration 1 μg/μL
- Pricing $495 USD
- Storage 4 ℃ (short-term) or -20 ℃ (long-term)
Terms and Licenses
-
Academic/Nonprofit Terms
-
Industry Terms
- Not Available to Industry
Quality Control
Every DNA prep is verified by next-generation sequencing, confirming plasmid identity, sequence integrity, and absence of DNA contaminants. Concentration is confirmed to be within ±2.5% of the stated concentration.
Our production process has been validated to consistently yield endotoxin levels below 1.0 EU/μg. Individual lots are not tested for endotoxin.
DNA (Catalog # 240425-D100)
Purpose
Ready-to-use, high-purity DNA prep. DNA aliquots are suitable for use in mammalian cell transfections and other molecular biology applications.
Delivery
- Amount 100 μg
- Concentration 1 μg/μL
- Pricing $585 USD
- Storage 4 ℃ (short-term) or -20 ℃ (long-term)
Terms and Licenses
-
Academic/Nonprofit Terms
-
Industry Terms
- Not Available to Industry
Quality Control
Every DNA prep is verified by next-generation sequencing, confirming plasmid identity, sequence integrity, and absence of DNA contaminants. Concentration is confirmed to be within ±2.5% of the stated concentration.
Our production process has been validated to consistently yield endotoxin levels below 1.0 EU/μg. Individual lots are not tested for endotoxin.
These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.
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For your Materials & Methods section:
HA-KIF5A-FRB was a gift from Anna Akhmanova (Addgene plasmid # 240425 ; http://n2t.net/addgene:240425 ; RRID:Addgene_240425) -
For your References section:
Optogenetic and chemical genetic tools for rapid repositioning of vimentin intermediate filaments. Pasolli M, Meiring JCM, Conboy JP, Koenderink GH, Akhmanova A. J Cell Biol. 2025 Sep 1;224(9):e202504004. doi: 10.1083/jcb.202504004. Epub 2025 Jul 8. 10.1083/jcb.202504004 PubMed 40627079
