CARMEN
(Plasmid
#240464)
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PurposeCompartment-specific measurements of Ca²⁺ dynamics by intensiometric Ca²⁺ biosensors targeted to mitochondria, cytosol and ER
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Depositing Lab
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Depositing OrganizationMedical University of Graz
Located in Austria -
Sequence Information
Ordering
| Item | Catalog # | Description | Quantity | Price (USD) | |
|---|---|---|---|---|---|
| Plasmid | 240464 | Standard format: Plasmid sent in bacteria as agar stab | 1 | $94 | |
| DNA | 240464-D50 | 50 μg of DNA in Tris buffer | $495 | ||
| DNA | 240464-D100 | 100 μg of DNA in Tris buffer | $585 | ||
Backbone
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Vector backbonepcDNA3.1(+)
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Backbone manufacturerGene Universal
- Backbone size w/o insert (bp) 5332
- Total vector size (bp) 10903
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Vector typeMammalian Expression
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Selectable markersNeomycin (select with G418)
Growth in Bacteria
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Bacterial Resistance(s)Ampicillin, 100 μg/mL
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Growth Temperature37°C
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Growth Strain(s)DH5alpha
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Copy numberHigh Copy
Gene/Insert
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Gene/Insert nameCARMEN
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Alt nameCEPIA3mt + T2A + mTagBFP2-NLS + P2A + NIR-GECO2G-NES + E2A + R-CEPIA1er
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SpeciesH. sapiens (human)
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Insert Size (bp)5571
Resource Information
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A portion of this plasmid was derived from a plasmid made byCommercially synthesized and cloned by Gene Universal
Terms and Licenses
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Academic/Nonprofit Terms
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Industry Terms
- Not Available to Industry
Trademarks:
- Zeocin® is an InvivoGen trademark.
Depositor Comments
Please visit https://doi.org/10.22541/au.174098553.38904074/v1 for preprint.
DNA (Catalog # 240464-D50)
Purpose
Ready-to-use, high-purity DNA prep. DNA aliquots are suitable for use in mammalian cell transfections and other molecular biology applications.
Delivery
- Amount 50 μg
- Concentration 1 μg/μL
- Pricing $495 USD
- Storage 4 ℃ (short-term) or -20 ℃ (long-term)
Terms and Licenses
-
Academic/Nonprofit Terms
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Industry Terms
- Not Available to Industry
Quality Control
Every DNA prep is verified by next-generation sequencing, confirming plasmid identity, sequence integrity, and absence of DNA contaminants. Concentration is confirmed to be within ±2.5% of the stated concentration.
Our production process has been validated to consistently yield endotoxin levels below 1.0 EU/μg. Individual lots are not tested for endotoxin.
DNA (Catalog # 240464-D100)
Purpose
Ready-to-use, high-purity DNA prep. DNA aliquots are suitable for use in mammalian cell transfections and other molecular biology applications.
Delivery
- Amount 100 μg
- Concentration 1 μg/μL
- Pricing $585 USD
- Storage 4 ℃ (short-term) or -20 ℃ (long-term)
Terms and Licenses
-
Academic/Nonprofit Terms
-
Industry Terms
- Not Available to Industry
Quality Control
Every DNA prep is verified by next-generation sequencing, confirming plasmid identity, sequence integrity, and absence of DNA contaminants. Concentration is confirmed to be within ±2.5% of the stated concentration.
Our production process has been validated to consistently yield endotoxin levels below 1.0 EU/μg. Individual lots are not tested for endotoxin.
These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.
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For your Materials & Methods section:
CARMEN was a gift from Roland Malli (Addgene plasmid # 240464 ; http://n2t.net/addgene:240464 ; RRID:Addgene_240464) -
For your References section:
A quad-cistronic fluorescent biosensor system for real-time detection of subcellular Ca(2+) signals. Lischnig A, Erdogan YC, Gottschalk B, Gruber M, Groselj-Strele A, Burgstaller S, Graier WF, Malli R. Br J Pharmacol. 2025 Oct 3. doi: 10.1111/bph.70211. 10.1111/bph.70211 PubMed 41039963
Map uploaded by the depositor.
