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FLAG-OXSR1 KM mutant
(Plasmid #240476)

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This material is available to academics and nonprofits only.
Item Catalog # Description Quantity Price (USD)
Plasmid 240476 Standard format: Plasmid sent in bacteria as agar stab 1 $94
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Backbone

  • Vector backbone
    pcDNA3
  • Vector type
    Mammalian Expression

Growth in Bacteria

  • Bacterial Resistance(s)
    Ampicillin, 100 μg/mL
  • Growth Temperature
    37°C
  • Growth Strain(s)
    DH5alpha
  • Copy number
    High Copy

Gene/Insert

  • Gene/Insert name
    OXSR1 (Oxidative Stress Responsive Kinase 1)
  • Species
    H. sapiens (human)
  • Insert Size (bp)
    1600
  • Mutation
    K46M mutation to create a kinase dead mutant
  • Entrez Gene
    OXSR1 (a.k.a. OSR1)
  • Tag / Fusion Protein
    • FLAG (N terminal on insert)

Cloning Information

  • Cloning method Restriction Enzyme
  • 5′ cloning site Uknown (unknown if destroyed)
  • 3′ cloning site Uknown (unknown if destroyed)

Resource Information

Terms and Licenses

  • Academic/Nonprofit Terms
  • Industry Terms
    • Not Available to Industry

Trademarks:

  • Zeocin® is an InvivoGen trademark.
How to cite this plasmid ( Back to top)

These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.

  • For your Materials & Methods section:

    FLAG-OXSR1 KM mutant was a gift from John Cusick & Hong-Bing Shu (Addgene plasmid # 240476 ; http://n2t.net/addgene:240476 ; RRID:Addgene_240476)

  • For your References section:

    Identification of RELT homologues that associate with RELT and are phosphorylated by OSR1. Cusick JK, Xu LG, Bin LH, Han KJ, Shu HB. Biochem Biophys Res Commun. 2006 Feb 10;340(2):535-43. doi: 10.1016/j.bbrc.2005.12.033. Epub 2005 Dec 19. 10.1016/j.bbrc.2005.12.033 PubMed 16389068
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