HA-RELT E deletion mutant
(Plasmid
#240477)
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PurposeExpression of RELT E deletion mutant in mammalian cells
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Depositing Labs
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Sequence Information
Ordering
| Item | Catalog # | Description | Quantity | Price (USD) | |
|---|---|---|---|---|---|
| Plasmid | 240477 | Standard format: Plasmid sent in bacteria as agar stab | 1 | $94 | |
Backbone
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Vector backbonepcDNA3
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Vector typeMammalian Expression
Growth in Bacteria
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Bacterial Resistance(s)Ampicillin, 100 μg/mL
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Growth Temperature37°C
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Growth Strain(s)DH5alpha
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Copy numberHigh Copy
Gene/Insert
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Gene/Insert nameRELT (Receptor Expressed in Lymphoid Tissues)
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SpeciesH. sapiens (human)
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Insert Size (bp)1200
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Mutation10 amino acids deleted from the carboxy-terminus of RELT
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Entrez GeneRELT (a.k.a. FLJ14993, TNFRSF19L)
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Tag
/ Fusion Protein
- 3xHA (N terminal on insert)
Cloning Information
- Cloning method Restriction Enzyme
- 5′ cloning site Uknown (unknown if destroyed)
- 3′ cloning site Uknown (unknown if destroyed)
Resource Information
Terms and Licenses
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Academic/Nonprofit Terms
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Industry Terms
- Not Available to Industry
Trademarks:
- Zeocin® is an InvivoGen trademark.
These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.
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For your Materials & Methods section:
HA-RELT E deletion mutant was a gift from John Cusick & Hong-Bing Shu (Addgene plasmid # 240477 ; http://n2t.net/addgene:240477 ; RRID:Addgene_240477) -
For your References section:
RELT family members activate p38 and induce apoptosis by a mechanism distinct from TNFR1. Moua P, Checketts M, Xu LG, Shu HB, Reyland ME, Cusick JK. Biochem Biophys Res Commun. 2017 Sep 9;491(1):25-32. doi: 10.1016/j.bbrc.2017.07.022. Epub 2017 Jul 5. 10.1016/j.bbrc.2017.07.022 PubMed 28688764