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pCS2+her3WT-P2A-sfGFP
(Plasmid #240545)

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This material is available to academics and nonprofits only.
Item Catalog # Description Quantity Price (USD)
Plasmid 240545 Standard format: Plasmid sent in bacteria as agar stab 1 $89
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Backbone

  • Vector backbone
    pCS2+MCS-P2A-sfGFP
  • Backbone manufacturer
    Jason Berman (Addgene Plasmid #74668)
  • Backbone size w/o insert (bp) 4915
  • Total vector size (bp) 5660
  • Modifications to backbone
    inserted her3 - wild type sequence (zebrafish ortholog to HES3)
  • Vector type
    Mammalian Expression

Growth in Bacteria

  • Bacterial Resistance(s)
    Ampicillin, 100 μg/mL
  • Growth Temperature
    37°C
  • Growth Strain(s)
    DH5alpha
  • Copy number
    Unknown

Gene/Insert

  • Gene/Insert name
    her3
  • Species
    D. rerio (zebrafish)
  • Insert Size (bp)
    1654
  • Entrez Gene
    her3 (a.k.a. HER-3, sb:eu263)

Terms and Licenses

  • Academic/Nonprofit Terms
  • Industry Terms
    • Not Available to Industry
Trademarks:
  • Zeocin® is an InvivoGen trademark.
How to cite this plasmid ( Back to top)

These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.

  • For your Materials & Methods section:

    pCS2+her3WT-P2A-sfGFP was a gift from Genevieve Kendall (Addgene plasmid # 240545 ; http://n2t.net/addgene:240545 ; RRID:Addgene_240545)

  • For your References section:

    Zebrafish her3 knockout impacts developmental and cancer-related gene signatures. Kent MR, Calderon D, Silvius KM, Kucinski JP, LaVigne CA, Cannon MV, Kendall GC. Dev Biol. 2023 Apr;496:1-14. doi: 10.1016/j.ydbio.2023.01.003. Epub 2023 Jan 23. 10.1016/j.ydbio.2023.01.003 PubMed 36696714
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