pIRES.Puro.mBeta
(Plasmid #24175)

• Depositing Lab: Robert Sobol
• Depositing Organization: University of Pittsburgh (University, School of Medicine, and Cancer Institute)
  Located in the United States of America
• Publication: Jelezcova et al Mutat Res. 2010 Apr 1;686(1-2):57-67. doi: 10.1016/j.mrfmmm.2010.01.016. Epub 2010 Jan 22.

Backbone

• Vector backbone: pIRESpuro-modified with gateway cassette by recombination clonin
• Backbone manufacturer: Clontech (Unmodified)
• Backbone size w/o insert (bp): 6000
• Vector type: Mammalian Expression
• Selectable markers: Puromycin

Growth in Bacteria

• Bacterial Resistance(s): Ampicillin, 100 μg/mL
• Growth Temperature: 37°C
• Growth Strain(s): DH5alpha
• Copy number: High Copy

Gene/Insert

• Gene/Insert name: pIRES.Puro.mBeta
• Alt name: Mouse Polymerase Beta
• Species: M. musculus (mouse)
• Insert Size (bp): 1008
• Mutation: x
• GenBank ID: NM_011130
• Entrez Gene: Polb (a.k.a. A430088C08Rik)

Cloning Information

• Cloning method: Gateway Cloning
• 5′ cloning site: x (not destroyed)
• 3′ cloning site: x (not destroyed)
• 5′ sequencing primer: x
• 3′ sequencing primer: x

Resource Information

• A portion of this plasmid was derived from a plasmid made by: x

Terms and Licenses

• Academic/Nonprofit Terms:
  • UBMTA
• Industry Terms:
  • Not Available to Industry

Depositor Comments

Mouse pol beta cDNA was cloned into pENTR-D (invitrogen). The mouse pol beta open reading frame was transferred from pENTRD-mPOL beta to a gateway modified pIRES-Puro plasmid via LR recombination reaction.

DNA (Catalog # 24175-D50)

Purpose

Ready-to-use, high-purity DNA prep. DNA aliquots are suitable for use in mammalian cell transfections and other molecular biology applications.

Delivery

• Amount: 50 μg
• Concentration: 1 μg/μL
• Pricing: $495 USD
• Storage: 4 ℃ (short-term) or -20 ℃ (long-term)

Terms and Licenses

• Academic/Nonprofit Terms:
  • UBMTA
• Industry Terms:
  • Not Available to Industry

Quality Control

Every DNA prep is verified by next-generation sequencing, confirming plasmid identity, sequence integrity, and absence of DNA contaminants. Concentration is confirmed to be within ±2.5% of the stated concentration.

Our production process has been validated to consistently yield endotoxin levels below 1.0 EU/μg. Individual lots are not tested for endotoxin.

DNA (Catalog # 24175-D100)

Purpose

Ready-to-use, high-purity DNA prep. DNA aliquots are suitable for use in mammalian cell transfections and other molecular biology applications.

Delivery

• Amount: 100 μg
• Concentration: 1 μg/μL
• Pricing: $585 USD
• Storage: 4 ℃ (short-term) or -20 ℃ (long-term)

Terms and Licenses

• Academic/Nonprofit Terms:
  • UBMTA
• Industry Terms:
  • Not Available to Industry

Quality Control

Every DNA prep is verified by next-generation sequencing, confirming plasmid identity, sequence integrity, and absence of DNA contaminants. Concentration is confirmed to be within ±2.5% of the stated concentration.

Our production process has been validated to consistently yield endotoxin levels below 1.0 EU/μg. Individual lots are not tested for endotoxin.