UAS:syp-dL5-mNeonGreen
(Plasmid
#241798)
-
PurposeExpresses synaptically localized dL5 protein fused to mNeonGreen, under control of UAS promoter
-
Depositing Lab
-
Depositing OrganizationNational Institute of Child Health and Human Development (NICHD)
Located in the United States of America -
Citations
-
Sequence Information
Ordering
| Item | Catalog # | Description | Quantity | Price (USD) | |
|---|---|---|---|---|---|
| Plasmid | 241798 | Standard format: Plasmid sent in bacteria as agar stab | 1 | $94 | |
| DNA | 241798-D50 | 50 μg of DNA in Tris buffer | $495 | ||
| DNA | 241798-D100 | 100 μg of DNA in Tris buffer | $585 | ||
Backbone
-
Vector backbonepT1UciMP
- Backbone size w/o insert (bp) 4547
- Total vector size (bp) 6981
-
Vector typeZebrafish transgenesis (tol1)
Growth in Bacteria
-
Bacterial Resistance(s)Ampicillin, 100 μg/mL
-
Growth Temperature37°C
-
Growth Strain(s)DH5alpha
-
Copy numberHigh Copy
Gene/Insert 1
-
Gene/Insert namesypb
-
Alt namesynaptophysinb
-
SpeciesD. rerio (zebrafish)
-
Insert Size (bp)891
-
Entrez Genesypb (a.k.a. zgc:136469, zgc:92837)
Gene/Insert 2
-
Gene/Insert namedL5
-
SpeciesSynthetic
-
Insert Size (bp)765
Gene/Insert 3
-
Gene/Insert namemNeonGreen (zebrafish codon optimized)
-
SpeciesSynthetic
-
Insert Size (bp)711
Resource Information
-
A portion of this plasmid was derived from a plasmid made byMichael Tsang, dL5-mCer cloned from Addgene plasmid #74116
Terms and Licenses
-
Academic/Nonprofit Terms
-
Industry Terms
- Not Available to Industry
Trademarks:
- Zeocin® is an InvivoGen trademark.
Depositor Comments
Please visit https://doi.org/10.1101/2025.04.04.647277 for bioRxiv preprint.
DNA (Catalog # 241798-D50)
Purpose
Ready-to-use, high-purity DNA prep. DNA aliquots are suitable for use in mammalian cell transfections and other molecular biology applications.
Delivery
- Amount 50 μg
- Concentration 1 μg/μL
- Pricing $495 USD
- Storage 4 ℃ (short-term) or -20 ℃ (long-term)
Terms and Licenses
-
Academic/Nonprofit Terms
-
Industry Terms
- Not Available to Industry
Quality Control
Every DNA prep is verified by next-generation sequencing, confirming plasmid identity, sequence integrity, and absence of DNA contaminants. Concentration is confirmed to be within ±2.5% of the stated concentration.
Our production process has been validated to consistently yield endotoxin levels below 1.0 EU/μg. Individual lots are not tested for endotoxin.
DNA (Catalog # 241798-D100)
Purpose
Ready-to-use, high-purity DNA prep. DNA aliquots are suitable for use in mammalian cell transfections and other molecular biology applications.
Delivery
- Amount 100 μg
- Concentration 1 μg/μL
- Pricing $585 USD
- Storage 4 ℃ (short-term) or -20 ℃ (long-term)
Terms and Licenses
-
Academic/Nonprofit Terms
-
Industry Terms
- Not Available to Industry
Quality Control
Every DNA prep is verified by next-generation sequencing, confirming plasmid identity, sequence integrity, and absence of DNA contaminants. Concentration is confirmed to be within ±2.5% of the stated concentration.
Our production process has been validated to consistently yield endotoxin levels below 1.0 EU/μg. Individual lots are not tested for endotoxin.
These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.
-
For your Materials & Methods section:
UAS:syp-dL5-mNeonGreen was a gift from Harold Burgess (Addgene plasmid # 241798 ; http://n2t.net/addgene:241798 ; RRID:Addgene_241798) -
For your References section:
Functional interrogation of neuronal connections by chemoptogenetic presynaptic ablation. Sharma H, Robea MA, McGrory NH, Bazan DC, Burton EA, Burgess HA. bioRxiv [Preprint]. 2025 Apr 5:2025.04.04.647277. doi: 10.1101/2025.04.04.647277. 10.1101/2025.04.04.647277 PubMed 40236196
Map uploaded by the depositor.
