pRP-BRG1-HA-SmBiT
(Plasmid
#241815)
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PurposeExpresses HA-tagged and HiBiT-tagged BRG1 for use with the Promega HiBiT system
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Depositing Lab
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Depositing OrganizationVanderbilt University
Located in United States of America -
Sequence Information
Ordering
| Item | Catalog # | Description | Quantity | Price (USD) | |
|---|---|---|---|---|---|
| Plasmid | 241815 | Standard format: Plasmid sent in bacteria as agar stab | 1 | $94 | |
| DNA | 241815-D50 | 50 μg of DNA in Tris buffer | $495 | ||
| DNA | 241815-D100 | 100 μg of DNA in Tris buffer | $585 | ||
Backbone
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Vector backbonepRP
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Backbone manufacturerVectorBuilder
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Vector typeMammalian Expression
Growth in Bacteria
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Bacterial Resistance(s)Ampicillin, 100 μg/mL
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Growth Temperature37°C
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Growth Strain(s)DH5alpha
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Copy numberUnknown
Gene/Insert
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Gene/Insert nameBRG1
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Alt nameSMARCA4
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SpeciesH. sapiens (human)
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Entrez GeneSMARCA4 (a.k.a. BAF190, BAF190A, BRG1, CSS4, MRD16, OTSC12, RTPS2, SNF2, SNF2-beta, SNF2L4, SNF2LB, SWI2, hSNF2b)
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Tags
/ Fusion Proteins
- HA
- SmBiT
Terms and Licenses
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Academic/Nonprofit Terms
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Industry Terms
- Not Available to Industry
Trademarks:
- Zeocin® is an InvivoGen trademark.
DNA (Catalog # 241815-D50)
Purpose
Ready-to-use, high-purity DNA prep. DNA aliquots are suitable for use in mammalian cell transfections and other molecular biology applications.
Delivery
- Amount 50 μg
- Concentration 1 μg/μL
- Pricing $495 USD
- Storage 4 ℃ (short-term) or -20 ℃ (long-term)
Terms and Licenses
-
Academic/Nonprofit Terms
-
Industry Terms
- Not Available to Industry
Quality Control
Every DNA prep is verified by next-generation sequencing, confirming plasmid identity, sequence integrity, and absence of DNA contaminants. Concentration is confirmed to be within ±2.5% of the stated concentration.
Our production process has been validated to consistently yield endotoxin levels below 1.0 EU/μg. Individual lots are not tested for endotoxin.
DNA (Catalog # 241815-D100)
Purpose
Ready-to-use, high-purity DNA prep. DNA aliquots are suitable for use in mammalian cell transfections and other molecular biology applications.
Delivery
- Amount 100 μg
- Concentration 1 μg/μL
- Pricing $585 USD
- Storage 4 ℃ (short-term) or -20 ℃ (long-term)
Terms and Licenses
-
Academic/Nonprofit Terms
-
Industry Terms
- Not Available to Industry
Quality Control
Every DNA prep is verified by next-generation sequencing, confirming plasmid identity, sequence integrity, and absence of DNA contaminants. Concentration is confirmed to be within ±2.5% of the stated concentration.
Our production process has been validated to consistently yield endotoxin levels below 1.0 EU/μg. Individual lots are not tested for endotoxin.
These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.
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For your Materials & Methods section:
pRP-BRG1-HA-SmBiT was a gift from Ethan Lee (Addgene plasmid # 241815 ; http://n2t.net/addgene:241815 ; RRID:Addgene_241815) -
For your References section:
The TRIP12 E3 ligase induces SWI/SNF component BRG1-beta-catenin interaction to promote Wnt signaling. Kassel S, Yuan K, Bunnag N, Neitzel LR, Lu W, Schwarzkopf A, Maines B, Loberg MA, Xu G, Adams A, McCray AD, Cho A, Rockouski M, Orton G, Goldsmith L, Aronno MMA, Spencer ZT, Khan OM, Ye F, Williams C, Lebensohn AM, Rohatgi R, Wang X, Weiss VL, Hong CC, Kettenbach AN, Robbins DJ, Ahmed Y, Lee E. Nat Commun. 2025 Jun 5;16(1):5248. doi: 10.1038/s41467-025-60535-5. 10.1038/s41467-025-60535-5 PubMed 40473626
