p131CB10-tphKAB
(Plasmid
#241911)
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PurposeTPA catabolic operon under the control of a PCA inducible biosensor
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Depositing Lab
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Depositing OrganizationUniversity of Manchester
Located in United Kingdom of Great Britain and Northern Ireland -
Sequence Information
Ordering
| Item | Catalog # | Description | Quantity | Price (USD) | |
|---|---|---|---|---|---|
| Plasmid | 241911 | Standard format: Plasmid sent in bacteria as agar stab | 1 | $94 | |
| DNA | 241911-D50 | 50 μg of DNA in Tris buffer | $495 | ||
| DNA | 241911-D100 | 100 μg of DNA in Tris buffer | $585 | ||
Backbone
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Vector backbonepSEVA131
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Vector typeSynthetic Biology
Growth in Bacteria
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Bacterial Resistance(s)Ampicillin, 100 μg/mL
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Growth Temperature37°C
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Growth Strain(s)DH5alpha
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Growth instructionsPlasmid can also be grown at 30 C, in NEB Stable cells, and in Pseudomonas putida KT2440. For growing in P. putida use ampicillin at 500 µg/ml.
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Copy numberHigh Copy
Gene/Insert
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Gene/Insert nametphK, tphA2, tphA3, tphB, tphA1
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SpeciesSynthetic
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Mutationnone
Cloning Information
- Cloning method Gibson Cloning
Resource Information
Terms and Licenses
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Academic/Nonprofit Terms
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Industry Terms
- Not Available to Industry
Trademarks:
- Zeocin® is an InvivoGen trademark.
DNA (Catalog # 241911-D50)
Purpose
Ready-to-use, high-purity DNA prep. DNA aliquots are suitable for use in mammalian cell transfections and other molecular biology applications.
Delivery
- Amount 50 μg
- Concentration 1 μg/μL
- Pricing $495 USD
- Storage 4 ℃ (short-term) or -20 ℃ (long-term)
Terms and Licenses
-
Academic/Nonprofit Terms
-
Industry Terms
- Not Available to Industry
Quality Control
Every DNA prep is verified by next-generation sequencing, confirming plasmid identity, sequence integrity, and absence of DNA contaminants. Concentration is confirmed to be within ±2.5% of the stated concentration.
Our production process has been validated to consistently yield endotoxin levels below 1.0 EU/μg. Individual lots are not tested for endotoxin.
DNA (Catalog # 241911-D100)
Purpose
Ready-to-use, high-purity DNA prep. DNA aliquots are suitable for use in mammalian cell transfections and other molecular biology applications.
Delivery
- Amount 100 μg
- Concentration 1 μg/μL
- Pricing $585 USD
- Storage 4 ℃ (short-term) or -20 ℃ (long-term)
Terms and Licenses
-
Academic/Nonprofit Terms
-
Industry Terms
- Not Available to Industry
Quality Control
Every DNA prep is verified by next-generation sequencing, confirming plasmid identity, sequence integrity, and absence of DNA contaminants. Concentration is confirmed to be within ±2.5% of the stated concentration.
Our production process has been validated to consistently yield endotoxin levels below 1.0 EU/μg. Individual lots are not tested for endotoxin.
These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.
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For your Materials & Methods section:
p131CB10-tphKAB was a gift from Neil Dixon (Addgene plasmid # 241911 ; http://n2t.net/addgene:241911 ; RRID:Addgene_241911) -
For your References section:
Complex waste stream valorization through combined enzymatic hydrolysis and catabolic assimilation by Pseudomonas putida. Chacon M, Alvarez-Gonzalez G, Gosalvitr P, Berepiki A, Fisher K, Cuellar-Franca R, Dixon N. Trends Biotechnol. 2025 Mar;43(3):647-672. doi: 10.1016/j.tibtech.2024.10.020. Epub 2024 Dec 4. 10.1016/j.tibtech.2024.10.020 PubMed 39638703
