pLX304-GNAQ Q209P-DCK-IRES-EGFP
(Plasmid
#241917)
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PurposeExpresses GNAQ Q209P-DCK mutant fusion protein
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Depositing Lab
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Publication
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Sequence Information
Ordering
| Item | Catalog # | Description | Quantity | Price (USD) | |
|---|---|---|---|---|---|
| Plasmid | 241917 | Standard format: Plasmid sent in bacteria as agar stab | 1 | $94 | |
Backbone
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Vector backbonepLX304
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Backbone manufacturerDavid Root (Addgene #25890)
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Vector typeMammalian Expression, Lentiviral
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Selectable markersBlasticidin
Growth in Bacteria
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Bacterial Resistance(s)Ampicillin, 100 μg/mL
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Growth Temperature37°C
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Growth Strain(s)NEB Stable
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Copy numberHigh Copy
Gene/Insert
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Gene/Insert nameGNAQ Q209P-DCK
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SpeciesH. sapiens (human)
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MutationQ209P
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Entrez GeneGNAQ (a.k.a. CMAL, CMC1, G-ALPHA-q, GAQ, SWS)
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Tag
/ Fusion Protein
- V5 (C terminal on insert)
Cloning Information
- Cloning method Gateway Cloning
Terms and Licenses
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Academic/Nonprofit Terms
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Industry Terms
- Not Available to Industry
Trademarks:
- Zeocin® is an InvivoGen trademark.
Depositor Comments
CAA -> CCA mutation to replace the Glutamine (Q209) with Proline (Q209P)
These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.
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For your Materials & Methods section:
pLX304-GNAQ Q209P-DCK-IRES-EGFP was a gift from Rizwan Haq (Addgene plasmid # 241917 ; http://n2t.net/addgene:241917 ; RRID:Addgene_241917)