pLenti-PGK-AP3S1 sgRES
(Plasmid
#241946)
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PurposeExpresses an AP3S1 guide RNA resistant AP3S1 protein
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Depositing Lab
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Publication
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Sequence Information
Ordering
| Item | Catalog # | Description | Quantity | Price (USD) | |
|---|---|---|---|---|---|
| Plasmid | 241946 | Standard format: Plasmid sent in bacteria as agar stab | 1 | $94 | |
Backbone
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Vector backbonepLENTI
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Vector typeMammalian Expression, Lentiviral
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Selectable markersHygromycin
Growth in Bacteria
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Bacterial Resistance(s)Ampicillin, 100 μg/mL
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Growth Temperature37°C
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Growth Strain(s)NEB Stable
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Copy numberHigh Copy
Gene/Insert
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Gene/Insert nameAP3S1 sgRES
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SpeciesH. sapiens (human)
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MutationsgRES
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Entrez GeneAP3S1 (a.k.a. CLAPS3, Sigma3A)
Cloning Information
- Cloning method Gateway Cloning
Terms and Licenses
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Academic/Nonprofit Terms
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Industry Terms
- Not Available to Industry
Trademarks:
- Zeocin® is an InvivoGen trademark.
Depositor Comments
CGG -> CGA silent mutation at PAM site for sgAP3S1 #3 (TCAACAACCACGGGAAGCCG) to make the AP3S1 cDNA resistant to sgAP3S1# 3
These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.
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For your Materials & Methods section:
pLenti-PGK-AP3S1 sgRES was a gift from Rizwan Haq (Addgene plasmid # 241946 ; http://n2t.net/addgene:241946 ; RRID:Addgene_241946)