AAV-PS Intein
(Plasmid #242020)

• Purpose: AAV version of PS Intein palsmid.
• Depositing Lab: Vladislav Verkhusha
• Publication: Baloban et al Nat Commun. 2025 Sep 11;16(1):8263. doi: 10.1038/s41467-025-63595-9.

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Backbone

• Vector backbone: pAAV-CW3SL-EGFP
• Backbone size w/o insert (bp): 3836
• Vector type: Mammalian Expression, Adenoviral

Growth in Bacteria

• Bacterial Resistance(s): Ampicillin, 100 μg/mL
• Growth Temperature: 37°C
• Growth Strain(s): NEB Stable
• Copy number: High Copy

Gene/Insert

• Gene/Insert name: PS Intein
• Alt name: mCherryN-SGY-IntN-IntC1-Linker 1-VVD-Linker 2-VVD-Linker 3-IntC-SSS-mCherryC--T2A-mEGFP
• Species: Synthetic
• Insert Size (bp): 2890
• Promoter: CMV
• Tag / Fusion Protein:
  • mEGFP (C terminal on insert)

Cloning Information

• Cloning method: Restriction Enzyme
• 5′ cloning site: NheI (not destroyed)
• 3′ cloning site: EcoRI (not destroyed)
• 5′ sequencing primer: CMV Forward
• 3′ sequencing primer: WPRE-R

Terms and Licenses

• Academic/Nonprofit Terms:
  • UBMTA
• Industry Terms:
  • Not Available to Industry