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pDest_PANCEd_AraC_sd2_R5_C1
(Plasmid #242150)

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This material is available to academics and nonprofits only.
Item Catalog # Description Quantity Price (USD)
Plasmid 242150 Standard format: Plasmid sent in bacteria as agar stab 1 $89
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Backbone

  • Vector backbone
    pDest
  • Vector type
    Bacterial Expression

Growth in Bacteria

  • Bacterial Resistance(s)
    Chloramphenicol, 25 μg/mL
  • Growth Temperature
    37°C
  • Growth Strain(s)
    DH5alpha
  • Copy number
    High Copy

Gene/Insert

  • Gene/Insert name
    AraC R5
  • Species
    Synthetic
  • Mutation
    A2E, L156I, R251H

Cloning Information

  • Cloning method Golden Gate

Resource Information

Terms and Licenses

  • Academic/Nonprofit Terms
  • Industry Terms
    • Not Available to Industry

Trademarks:

  • Zeocin® is an InvivoGen trademark.

Depositor Comments

Please visit https://doi.org/10.1101/2025.06.12.659331 for bioRxiv preprint.

How to cite this plasmid ( Back to top)

These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.

  • For your Materials & Methods section:

    pDest_PANCEd_AraC_sd2_R5_C1 was a gift from Dominik Niopek (Addgene plasmid # 242150 ; http://n2t.net/addgene:242150 ; RRID:Addgene_242150)

  • For your References section:

    Phage-assisted evolution of allosteric protein switches. Southern NT, von Bachmann A, Hovsepyan A, Griebl M, Wolf B, Lemmen N, Kroell AS, Westermann S, Mathony J, Niopek D. Nat Commun. 2026 Apr 14;17(1):3498. doi: 10.1038/s41467-026-71717-0. 10.1038/s41467-026-71717-0 PubMed 41980963
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