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pFastBac1-His-MuSK-homo-S752D-530-869
(Plasmid #242443)

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This material is available to academics and nonprofits only.
Item Catalog # Description Quantity Price (USD)
Plasmid 242443 Standard format: Plasmid sent in bacteria as agar stab 1 $94
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Backbone

  • Vector backbone
    pFastBac1
  • Backbone manufacturer
    Invitrogen
  • Backbone size w/o insert (bp) 4837
  • Total vector size (bp) 5860
  • Vector type
    Bacterial Expression

Growth in Bacteria

  • Bacterial Resistance(s)
    Ampicillin, 100 μg/mL
  • Growth Temperature
    37°C
  • Growth Strain(s)
    DH5alpha
  • Growth instructions
    Bac-to-bac system
  • Copy number
    High Copy

Gene/Insert

  • Gene/Insert name
    Muscle specific kinase JM and TKD
  • Species
    H. sapiens (human)
  • Insert Size (bp)
    1023
  • Mutation
    aa 530-869 only; changed Serine 752 to Aspartic acid
  • GenBank ID
    NM_005592
  • Entrez Gene
    MUSK (a.k.a. CMS9, FADS, FADS1)
  • Promoter polydherin
  • Tag / Fusion Protein
    • 6xHis tag (N terminal on insert)

Cloning Information

  • Cloning method Restriction Enzyme
  • 5′ cloning site NcoI (not destroyed)
  • 3′ cloning site XhoI (not destroyed)
  • 5′ sequencing primer GGATTATTCATACCGTCCCACCATCG
  • (Common Sequencing Primers)

Resource Information

  • A portion of this plasmid was derived from a plasmid made by
    I.M.A.G.E. consortium. See Strausberg et al (2002) Mammalian Gene Collection Program Team. Generation and initial analysis of more than 15,000 full-length human and mouse cDNA sequences. Proc Natl Acad Sci U S A. 2002 Dec 24;99(26):16899-903. doi: 10.1073/pnas.242603899. PMID: 12477932; PMCID: PMC139241.

Terms and Licenses

  • Academic/Nonprofit Terms
  • Industry Terms
    • Not Available to Industry

Trademarks:

  • Zeocin® is an InvivoGen trademark.
How to cite this plasmid ( Back to top)

These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.

  • For your Materials & Methods section:

    pFastBac1-His-MuSK-homo-S752D-530-869 was a gift from Mark Lemmon (Addgene plasmid # 242443 ; http://n2t.net/addgene:242443 ; RRID:Addgene_242443)

  • For your References section:

    An S752D activation loop mutation dynamically primes Muscle-Specific Kinase for activation. Promer JJ, Murphy JW, Lemmon MA, Tsutsui Y, Herbst R. Biochem J. 2026 Jul 8;483(7):1221-1235. doi: 10.1042/BCJ20260159. 10.1042/BCJ20260159 PubMed 42240394
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