SFFV-NFKBIA-dTomato
(Plasmid
#242697)
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PurposeValidation of factors for immature cDC1 reprogramming fidelity. Lentiviral expresison of NFKBIA with dTomato.
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Depositing Lab
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Sequence Information
Ordering
| Item | Catalog # | Description | Quantity | Price (USD) | |
|---|---|---|---|---|---|
| Plasmid | 242697 | Standard format: Plasmid sent in bacteria as agar stab | 1 | $94 | |
Backbone
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Vector backboneSFFV-MCS-dTomato
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Vector typeLentiviral
Growth in Bacteria
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Bacterial Resistance(s)Ampicillin, 100 μg/mL
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Growth Temperature37°C
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Growth Strain(s)NEB Stable
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Copy numberHigh Copy
Gene/Insert
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Gene/Insert nameNFKBIA
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SpeciesH. sapiens (human)
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Insert Size (bp)954
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Entrez GeneNFKBIA (a.k.a. EDAID2, IKBA, MAD-3, NFKBI)
- Promoter SFFV (spleen focus-forming virus) promoter
Cloning Information
- Cloning method Restriction Enzyme
- 5′ cloning site unknown (unknown if destroyed)
Resource Information
Terms and Licenses
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Academic/Nonprofit Terms
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Industry Terms
- Not Available to Industry
Trademarks:
- Zeocin® is an InvivoGen trademark.
Depositor Comments
Also expresses dTomato from IRES site
These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.
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For your Materials & Methods section:
SFFV-NFKBIA-dTomato was a gift from Filipe Pereira (Addgene plasmid # 242697 ; http://n2t.net/addgene:242697 ; RRID:Addgene_242697) -
For your References section:
A combinatorial transcription factor screening platform for immune cell reprogramming. Kurochkin I, Altman AR, Caiado I, Pertiga-Cabral D, Halitzki E, Minaeva M, Zimmermannova O, Henriques-Oliveira L, Klein D, Nair M, Oliveira D, Cajal LR, Knittel R, Feick C, Ringner M, Martin M, Cirovic B, Pires CF, Rosa FF, Sitnicka E, Theis FJ, Pereira CF. Cell Syst. 2026 Jan 21;17(1):101457. doi: 10.1016/j.cels.2025.101457. Epub 2026 Jan 14. 10.1016/j.cels.2025.101457 PubMed 41539305