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pENTR-SpCas9
(Plasmid #242844)

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This material is available to academics and nonprofits only.
Item Catalog # Description Quantity Price (USD)
Plasmid 242844 Standard format: Plasmid sent in bacteria as agar stab 1 $94
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Backbone

  • Vector backbone
    pENTR4-FLAG (w210-2)
  • Backbone manufacturer
    Eric Campeau and Paul Kaufman, Addgene plasmid #17423
  • Backbone size w/o insert (bp) 2333
  • Total vector size (bp) 6472
  • Vector type
    Bacterial Expression, CRISPR, Gateway Cloning, Synthetic Biology

Growth in Bacteria

  • Bacterial Resistance(s)
    Kanamycin, 50 μg/mL
  • Growth Temperature
    37°C
  • Growth Strain(s)
    DH5alpha
  • Copy number
    Low Copy

Gene/Insert

  • Gene/Insert name
    Cas9
  • Alt name
    SpCas9
  • Species
    S. pyogenes
  • Insert Size (bp)
    4101
  • Entrez Gene
    cas9 (a.k.a. SD90_RS03825, SD90_03830)
  • Tag / Fusion Protein
    • Flag (C terminal on insert)

Terms and Licenses

  • Academic/Nonprofit Terms
  • Industry Terms
    • Not Available to Industry

Trademarks:

  • Zeocin® is an InvivoGen trademark.

Depositor Comments

Cas9 insert was cloned into pENTR4-FLAG using NEBuilder HIFI DNA assembly

How to cite this plasmid ( Back to top)

These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.

  • For your Materials & Methods section:

    pENTR-SpCas9 was a gift from Siyuan Zhang (Addgene plasmid # 242844 ; http://n2t.net/addgene:242844 ; RRID:Addgene_242844)

  • For your References section:

    Tumor-intrinsic IFNγ signaling and niche adaptation drive early colonization in ovarian cancer metastasis. Aleksandrovic E, Fross SR, Golomb SM, Ma W, Liu X, Zhao Z, Das NM, Reese TC, Borges HM, Tenekeci A, Yu L, Lopez J, Zhao M, Zhong Z, Dean KM, Lea J, Xu L, Stack MS, Zhang S. Nat Commun. 2026 Sep 3;17(1):10497. doi: 10.1038/s41467-026-77222-8. 10.1038/s41467-026-77222-8 PubMed 42834039
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