RADARS_goldengate_mNeon
(Plasmid #242850)

• Purpose: RADARS backbone with BsaI golden gate cloning site. Expresses constitutive mCherry and RADARS guide controlled mNeon payload. Lentivirus backbone with IRES_puromycin for convenient transduction.
• Depositing Lab: Fei Chen
• Depositing Organization: Broad Institute
  Located in the United States of America
• Publication: Koob et al Nat Protoc. 2026 Apr 2. doi: 10.1038/s41596-025-01305-x.

Backbone

• Vector backbone: pUC19
• Backbone manufacturer: NEB
• Backbone size w/o insert (bp): 3000
• Total vector size (bp): 8391
• Modifications to backbone: EF1a promotoer, mCherry ORF, T2A, BsaI golden gate site, P2A, mNeon, IRES, Puromycin R, bGH_polyA
• Vector type: Mammalian Expression, Synthetic Biology
• Selectable markers: Puromycin ; mCherry

Growth in Bacteria

• Bacterial Resistance(s): Ampicillin, 100 μg/mL
• Growth Temperature: 37°C
• Growth Strain(s): DH5alpha
• Copy number: Unknown

Gene/Insert 1

• Gene/Insert name: mCherry
• Species: Synthetic
• Insert Size (bp): 705
• GenBank ID: AY678264.1

Gene/Insert 2

• Gene/Insert name: mNeon
• Insert Size (bp): 707
• Mutation: no start codon
• GenBank ID: LC279210.1

Gene/Insert 3

• Gene/Insert name: Internal Ribosome Entry Site (IRES)

Gene/Insert 4

• Gene/Insert name: Puromycin (PuroR)

Resource Information

• A portion of this plasmid was derived from a plasmid made by: Cloned in collaboration with Kaiyi Jiang of the AbuGoot lab at MIT

Terms and Licenses

• Academic/Nonprofit Terms:
  • UBMTA
  • mNeonGreen Label License
• Industry Terms:
  • Not Available to Industry