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PMaCTID
(Plasmid #243000)

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This material is available to academics and nonprofits only.
Item Catalog # Description Quantity Price (USD)
Plasmid 243000 Standard format: Plasmid sent in bacteria as agar stab 1 $94
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Backbone

  • Vector backbone
    pFA6a
  • Total vector size (bp) 5083
  • Vector type
    PCR template
  • Selectable markers
    Puromycin

Growth in Bacteria

  • Bacterial Resistance(s)
    Ampicillin, 100 μg/mL
  • Growth Temperature
    37°C
  • Growth Strain(s)
    DH5alpha
  • Copy number
    High Copy

Gene/Insert

  • Gene/Insert name
    TurboID
  • Species
    BirA mutant
  • Insert Size (bp)
    961
  • Mutation
    Q65P, I87V, R118S, E140K, Q141R, S150G, L151P, V160A, T192A, K194I, M209V, M241T, S263P, I305V
  • Tag / Fusion Protein
    • HA tag (C terminal on insert)

Cloning Information

  • Cloning method Restriction Enzyme
  • 5′ cloning site BamHI (not destroyed)
  • 3′ cloning site SpeI (not destroyed)
  • 5′ sequencing primer SP6
  • (Common Sequencing Primers)

Resource Information

  • A portion of this plasmid was derived from a plasmid made by
    Alice Ting; TurboID was cloned from plasmid 3xHA-TurboID-NLS_pcDNA3 (Addgene #107171).

Terms and Licenses

  • Academic/Nonprofit Terms
  • Industry Terms
    • Not Available to Industry

Trademarks:

  • Zeocin® is an InvivoGen trademark.
How to cite this plasmid ( Back to top)

These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.

  • For your Materials & Methods section:

    PMaCTID was a gift from Stefan Koch (Addgene plasmid # 243000 ; http://n2t.net/addgene:243000 ; RRID:Addgene_243000)

  • For your References section:

    Comprehensive proximity proteomics expand the known interactome of the oncoprotein beta-catenin. Moparthi L, Zhong W, Koch S. Sci Rep. 2026 Sep 1;16(1):27374. doi: 10.1038/s41598-026-68008-5. 10.1038/s41598-026-68008-5 PubMed 42680804
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