|Item||Catalog #||Description||Quantity||Price (USD)|
|Plasmid||24370||Standard format: Plasmid sent in bacteria as agar stab||1||$75|
This material is available to academics and nonprofits only.
Vector backbonepBluescript SK (+)
- Backbone size w/o insert (bp) 2950
Vector typeBacterial Expression
Growth in Bacteria
Insert Size (bp)2750
- Cloning method Restriction Enzyme
- 5′ cloning site EcoRI (unknown if destroyed)
- 3′ cloning site XbaI (unknown if destroyed)
- 5′ sequencing primer T7
- 3′ sequencing primer M13-pUC-rev (Common Sequencing Primers)
QS ORF was PCR amplified from pAC-QS to include EcoRI/XbaI restriction sites.
These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.
For your Materials & Methods section:pBS-SK-QS was a gift from Liqun Luo (Addgene plasmid # 24370 ; http://n2t.net/addgene:24370 ; RRID:Addgene_24370)
For your References section:The Q System: A Repressible Binary System for Transgene Expression, Lineage Tracing, and Mosaic Analysis. Potter CJ, Tasic B, Russler EV, Liang L, Luo L.. Volume 141, Issue 3, 536-548, 30 April 2010 10.1016/j.cell.2010.02.025 PubMed 20434990