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L4802 VEGFR2 NTEVp chain (WT NTEVp) in PolyTX-mNeonGreen
(Plasmid #244173)

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This material is available to academics and nonprofits only.
Item Catalog # Description Quantity Price (USD)
Plasmid 244173 Standard format: Plasmid sent in bacteria as agar stab 1 $94
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DNA 244173-D50 50 μg of DNA in Tris buffer $495
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DNA 244173-D100 100 μg of DNA in Tris buffer $585
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Backbone

  • Vector backbone
    pPD1054 Polytransfect L1 mNeonGreen
  • Vector type
    Mammalian Expression, Synthetic Biology

Growth in Bacteria

  • Bacterial Resistance(s)
    Kanamycin, 50 μg/mL
  • Growth Temperature
    30°C
  • Growth Strain(s)
    NEB Stable
  • Copy number
    High Copy

Gene/Insert

  • Gene/Insert name
    MESA NTEVp chain with human VEGFR2 SS, ECD and TMD, and NTEVp (WT)
  • Species
    H. sapiens (human), Synthetic
  • Entrez Gene
    KDR (a.k.a. CD309, FLK1, VEGFR, VEGFR2)
  • Promoter hEF1a
  • Tag / Fusion Protein
    • VEGFR2 signal peptide - 3xFLAG (N terminal on insert)

Cloning Information

  • Cloning method Restriction Enzyme
  • 5′ cloning site Unknown (unknown if destroyed)

Terms and Licenses

Trademarks:

  • Zeocin® is an InvivoGen trademark.

Depositor Comments

Please visit https://doi.org/10.1101/2024.03.23.586421 for bioRxiv preprint.

Purpose

Ready-to-use, high-purity DNA prep. DNA aliquots are suitable for use in mammalian cell transfections and other molecular biology applications.

Delivery

  • Amount 50 μg
  • Concentration 1 μg/μL
  • Pricing $495 USD
  • Storage 4 ℃ (short-term) or -20 ℃ (long-term)

Terms and Licenses

Quality Control

Every DNA prep is verified by next-generation sequencing, confirming plasmid identity, sequence integrity, and absence of DNA contaminants. Concentration is confirmed to be within ±2.5% of the stated concentration.

Our production process has been validated to consistently yield endotoxin levels below 1.0 EU/μg. Individual lots are not tested for endotoxin.

Purpose

Ready-to-use, high-purity DNA prep. DNA aliquots are suitable for use in mammalian cell transfections and other molecular biology applications.

Delivery

  • Amount 100 μg
  • Concentration 1 μg/μL
  • Pricing $585 USD
  • Storage 4 ℃ (short-term) or -20 ℃ (long-term)

Terms and Licenses

Quality Control

Every DNA prep is verified by next-generation sequencing, confirming plasmid identity, sequence integrity, and absence of DNA contaminants. Concentration is confirmed to be within ±2.5% of the stated concentration.

Our production process has been validated to consistently yield endotoxin levels below 1.0 EU/μg. Individual lots are not tested for endotoxin.

How to cite this plasmid ( Back to top)

These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.

  • For your Materials & Methods section:

    L4802 VEGFR2 NTEVp chain (WT NTEVp) in PolyTX-mNeonGreen was a gift from Joshua Leonard (Addgene plasmid # 244173 ; http://n2t.net/addgene:244173 ; RRID:Addgene_244173)

  • For your References section:

    Conversion of natural cytokine receptors into orthogonal synthetic biosensors. Edelstein HI, Cosio A, Ezekiel ML, Corcoran WK, Morris AH, Leonard JN. Nat Chem Biol. 2025 Aug 22:10.1038/s41589-025-01986-1. doi: 10.1038/s41589-025-01986-1. 10.1038/s41589-025-01986-1 PubMed 40846994
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