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LifeAct-frFAST
(Plasmid #244247)

Ordering

This material is available to academics and nonprofits only.
Item Catalog # Description Quantity Price (USD)
Plasmid 244247 Standard format: Plasmid sent in bacteria as agar stab 1 $94

Backbone

  • Vector backbone
    pIRES
  • Vector type
    Mammalian Expression
  • Selectable markers
    Neomycin (select with G418)

Growth in Bacteria

  • Bacterial Resistance(s)
    Kanamycin, 50 μg/mL
  • Growth Temperature
    37°C
  • Growth Strain(s)
    DH5alpha
  • Copy number
    High Copy

Gene/Insert

  • Gene/Insert name
    LifeAct-frFAST
  • Species
    Synthetic

Terms and Licenses

  • Academic/Nonprofit Terms
  • Industry Terms
    • Not Available to Industry

Trademarks:

  • Zeocin® is an InvivoGen trademark.

Depositor Comments

Please visit https://doi.org/10.26434/chemrxiv-2025-k7vd4 for ChemRxiv preprint.

How to cite this plasmid ( Back to top)

These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.

  • For your Materials & Methods section:

    LifeAct-frFAST was a gift from Péter Kele (Addgene plasmid # 244247 ; http://n2t.net/addgene:244247 ; RRID:Addgene_244247)
  • For your References section:

    Evaluating the far-red emitting fluorescence-activating and absorption-shifting tag (frFAST) chemogenetic labeling system for live-cell super-resolution microscopy. Kozma E, Novak T, Szatmari A, Torok G, Nikic-Spiegel I, Cserep GB, Nemeth K, Czvik E, Erdelyi M, Kele P. Methods Appl Fluoresc. 2026 Sep 8;14(4). doi: 10.1088/2050-6120/ae9fb8. 10.1088/2050-6120/ae9fb8 PubMed 42660196