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(Plasmid #24506)


Full plasmid sequence is not available for this item.


Item Catalog # Description Quantity Price (USD)
Plasmid 24506 Standard format: Plasmid sent in bacteria as agar stab 1 $85

This material is available to academics and nonprofits only.


  • Vector backbone
    Yeplac 181
  • Backbone size w/o insert (bp) 5741
  • Vector type
    Yeast Expression
  • Selectable markers

Growth in Bacteria

  • Bacterial Resistance(s)
    Ampicillin, 100 μg/mL
  • Growth Temperature
  • Growth Strain(s)
  • Copy number
    High Copy


  • Gene/Insert name
  • Species
    S. cerevisiae (budding yeast)
  • Entrez Gene
    UBR1 (a.k.a. YGR184C, PTR1)
  • Tag / Fusion Protein
    • FLAG (N terminal on insert)

Cloning Information

  • Cloning method Restriction Enzyme
  • 5′ cloning site unknown (unknown if destroyed)
  • 3′ cloning site unknown (unknown if destroyed)
  • 5′ sequencing primer yADH1-F
  • 3′ sequencing primer M13pUC-Rev
  • (Common Sequencing Primers)

Resource Information

Terms and Licenses

  • Academic/Nonprofit Terms
  • Industry Terms
    • Not Available to Industry
  • Zeocin® is an InvivoGen trademark.

Depositor Comments

SBX stands for SalI, BamHI, XhoI. SalI is 15 bp before ATG codon of UBR1ORF. BamHI and XhoI are right after the stop codon of the UBR1ORF.
The plasmid expresses UBR1 which is N-terminally Flag tagged and has "clean" C-terminus.
Note that it does not have NcoI site upstream of ATG codon. Active in degrading N-end rule substrates.

How to cite this plasmid ( Back to top)

These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.

  • For your Materials & Methods section:

    pFLAG UBR1 SBX was a gift from Alexander Varshavsky (Addgene plasmid # 24506 ; ; RRID:Addgene_24506)
  • For your References section:

    Substrate-binding sites of UBR1, the ubiquitin ligase of the N-end rule pathway. Xia Z, Webster A, Du F, Piatkov K, Ghislain M, Varshavsky A. J Biol Chem. 2008 Aug 29. 283(35):24011-28. 10.1074/jbc.M802583200 PubMed 18566452