pENTR-Site6
(Plasmid
#24515)
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Depositing Lab
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Publication
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Sequence Information
Ordering
| Item | Catalog # | Description | Quantity | Price (USD) | |
|---|---|---|---|---|---|
| Plasmid | 24515 | Standard format: Plasmid sent in bacteria as agar stab | 1 | $85 | |
This material is available to academics and nonprofits only.
Backbone
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Vector backbonepDONR221
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Backbone manufacturerInvitrogen
- Backbone size w/o insert (bp) 2538
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Vector typeMammalian Expression, Mouse Targeting, Cre/Lox
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Selectable markersGentamicin
Growth in Bacteria
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Bacterial Resistance(s)Kanamycin, 50 μg/mL
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Growth Temperature37°C
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Growth Strain(s)DH5alpha
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Copy numberHigh Copy
Gene/Insert
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Gene/Insert nameSplit RFP-GFP chimera
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SpeciesAequorea victoria and Discosoma sp. reef coral
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Insert Size (bp)2984
Cloning Information
- Cloning method Gateway Cloning
- 5′ cloning site attL1 (not destroyed)
- 3′ cloning site attL2 (not destroyed)
- 5′ sequencing primer M13 forward
- 3′ sequencing primer M13 reverse (Common Sequencing Primers)
Terms and Licenses
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Academic/Nonprofit Terms
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Industry Terms
- Not Available to Industry
Trademarks:
- Zeocin® is an InvivoGen trademark.
How to cite this plasmid
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These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.
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For your Materials & Methods section:
pENTR-Site6 was a gift from Kazuo Kinoshita (Addgene plasmid # 24515 ; http://n2t.net/addgene:24515 ; RRID:Addgene_24515) -
For your References section:
Chromosomal manipulation by site-specific recombinases and fluorescent protein-based vectors. Uemura M, Niwa Y, Kakazu N, Adachi N, Kinoshita K. PLoS One. 2010 . 5(3):e9846. 10.1371/journal.pone.0009846 PubMed 20352097
Map uploaded by the depositor.
