Skip to main content
This website uses cookies to ensure you get the best experience. By continuing to use this site, you agree to the use of cookies.

Please note: Your browser does not support the features used on Addgene's website. You may not be able to create an account or request plasmids through this website until you upgrade your browser. Learn more

Please note: Your browser does not fully support some of the features used on Addgene's website. If you run into any problems registering, depositing, or ordering please contact us at [email protected] Learn more

pENTR-PCBP1
(Plasmid #16177)

Loading...

Full plasmid sequence is not available for this item.

Ordering

Item Catalog # Description Quantity Price (USD)
Plasmid 16177 Standard format: Plasmid sent in bacteria as agar stab 1 $65

This material is available to academics and nonprofits only.

Backbone

Growth in Bacteria

  • Bacterial Resistance(s)
    Spectinomycin
  • Growth Temperature
    37°C
  • Growth Strain(s)
    DH5alpha
  • Copy number
    High Copy

Gene/Insert

  • Gene/Insert name
    Poly(rC) binding protein 1
  • Alt name
    PCBP1
  • Species
    H. sapiens (human)
  • Insert Size (bp)
    1068
  • Entrez Gene
    PCBP1 (a.k.a. HEL-S-85, HNRPE1, HNRPX, hnRNP-E1, hnRNP-X)

Cloning Information

  • Cloning method Gateway Cloning
  • 5′ cloning site attP1 (destroyed during cloning)
  • 3′ cloning site attP2 (destroyed during cloning)
  • 5′ sequencing primer M13F
  • 3′ sequencing primer M13R
  • (Common Sequencing Primers)

Resource Information

  • Terms and Licenses

Depositor Comments

Full-length human cDNAs were amplified using PCR primers and cloned into the pDONR223 "Donor" vector using the Gateway system as described (Rual et al., 2004), resulting in "Entry" clones. Reverse primers do not include stop codons. Forward primer: 5'-GGGGACAACTTTGTACAAAAAAGTTGGC ATGGATGCCGGTGTGACTGA-3'. Reverse primer: 5'-GGGGACAACTTTGTACAAGAAAGTTGG GCTGCACCCCATGCCCTTC-3'.

How to cite this plasmid ( Back to top)

These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.

  • For your Materials & Methods section:

    pENTR-PCBP1 was a gift from Huda Zoghbi (Addgene plasmid # 16177 ; http://n2t.net/addgene:16177 ; RRID:Addgene_16177)
  • For your References section:

    A protein-protein interaction network for human inherited ataxias and disorders of Purkinje cell degeneration. Lim J, Hao T, Shaw C, Patel AJ, Szabo G, Rual JF, Fisk CJ, Li N, Smolyar A, Hill DE, Barabasi AL, Vidal M, Zoghbi HY. Cell. 2006 May 19. 125(4):801-14. 10.1016/j.cell.2006.03.032 PubMed 16713569