pFB-CMV-DIO-MTS-roGFP-WPRE
(Plasmid #245375)

• Purpose: Cre-dependent expression of a green fluorescent protein biosensor to determine mitochondrial cellular reduction-oxidation (redox) states.
• Depositing Lab: D. James Surmeier
• Publication: Tubert et al Neurobiol Dis. 2023 Nov;188:106328. doi: 10.1016/j.nbd.2023.106328. Epub 2023 Oct 16.

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Backbone

• Vector backbone: pFB
• Backbone manufacturer: Virovek
• Backbone size w/o insert (bp): 4838
• Total vector size (bp): 7381
• Vector type: Mammalian Expression, AAV

Growth in Bacteria

• Bacterial Resistance(s): Ampicillin, 100 μg/mL
• Growth Temperature: 37°C
• Growth Strain(s): NEB Stable
• Copy number: Unknown

Gene/Insert

• Gene/Insert name: roGFP
• Species: Synthetic
• Insert Size (bp): 2543
• Promoter: CMV
• Tag / Fusion Protein:
  • mitochondrial-targeting sequence of the S. pombe cytochrome c oxidase subunit IV (cox4) (N terminal on insert)

Cloning Information

• Cloning method: Unknown
• 5′ sequencing primer: CGCAAATGGGCGGTAGGCGTG
• 3′ sequencing primer: GAAATTTGTGATGCTATTGC

Terms and Licenses

• Academic/Nonprofit Terms:
  • UBMTA
• Industry Terms:
  • Not Available to Industry