|Item||Catalog #||Description||Quantity||Price (USD)|
|Plasmid||24584||Standard format: Plasmid sent in bacteria as agar stab||1||$65|
This material is available to academics and nonprofits only.
- Backbone size w/o insert (bp) 6000
Vector typeYeast Expression
Growth in Bacteria
Copy numberHigh Copy
Gene/Insert namepADH1 yeCherry::pTEF1 (S.C.)yeGFP-DHFR
Alt nameADH1 promoter ye Cherry
Alt nameTEF1 (S.C.) promoter yeGFP-ecDHFR
SpeciesS. cerevisiae (budding yeast)
Insert Size (bp)3060
Mutation1. Truncated version of ADH700 promoter (SbfI/SpeI) driven yeast enhanced RFP (SpeI/-). 2. promoter TEF1(S.C.) (SacII/BglII) driven yeast enhanced GFP fused to E.coli DHFR (BglII/XhoI)
- Cloning method Restriction Enzyme
- 5′ cloning site sbf1 (not destroyed)
- 3′ cloning site XhoI (not destroyed)
- 5′ sequencing primer n/a (Common Sequencing Primers)
The TEF1 (S.C.) promoter region has 4 base differences from the published sequences but the authors report that they do not affect transcriptional function.
These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.
For your Materials & Methods section:pGADT7-ADH700-yeCherry-pTEF1 (S.C.)-yeGFP-DHFR was a gift from Thomas Wandless (Addgene plasmid # 24584 ; http://n2t.net/addgene:24584 ; RRID:Addgene_24584)
For your References section:Dicistronic regulation of fluorescent proteins in the budding yeast Saccharomyces cerevisiae. Edwards SR, Wandless TJ. Yeast. 2009 Dec 17. ():. 10.1002/yea.1744 PubMed 20017217