CCT8-pcDNA3.1(+)-N-eGFP
(Plasmid
#245884)
-
PurposeExpress human CCT8 tagged with eGFP in Nter
-
Depositing Lab
-
Depositing OrganizationTurku Bioscience Centre
Located in Finland -
Publication
-
Sequence Information
Ordering
| Item | Catalog # | Description | Quantity | Price (USD) | |
|---|---|---|---|---|---|
| Plasmid | 245884 | Standard format: Plasmid sent in bacteria as agar stab | 1 | $94 | |
Backbone
-
Vector backbonepcDNA3.1(+)-C-eGFP
-
Vector typeMammalian Expression
Growth in Bacteria
-
Bacterial Resistance(s)Ampicillin, 100 μg/mL
-
Growth Temperature37°C
-
Growth Strain(s)DH5alpha
-
Copy numberUnknown
Gene/Insert
-
Gene/Insert nameCCT8
-
SpeciesH. sapiens (human)
-
Entrez GeneCCT8 (a.k.a. C21orf112, Cctq, D21S246, PRED71)
-
Tag
/ Fusion Protein
- eGFP
Cloning Information
- Cloning method Gene Synthesis
- 5′ sequencing primer cmv
- (Common Sequencing Primers)
Resource Information
Terms and Licenses
-
Academic/Nonprofit Terms
-
Industry Terms
- Not Available to Industry
Trademarks:
- Zeocin® is an InvivoGen trademark.
Depositor Comments
The CCT8-pcDNA3.1(+)-N-eGFP construct was purchased from Genscript. Briefly, the gene fragment was synthesized using gene synthesis and cloned into pcDNA3.1(+)-N-eGFP at the BamHI/XhoI sites. Please visit https://doi.org/10.64898/2025.12.03.691809 for bioRxiv preprint.
These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.
-
For your Materials & Methods section:
CCT8-pcDNA3.1(+)-N-eGFP was a gift from Guillaume Jacquemet (Addgene plasmid # 245884 ; http://n2t.net/addgene:245884 ; RRID:Addgene_245884) -
For your References section:
CCT8 associates with the MYO10 motor domain and regulates filopodia and breast cancer cell invasion. Popović A, Ojalill M, Touma C, Ball NJ, Miihkinen M, Joshi O, Dibus M, Pylvänäinen J, Ivaska J, Goult BT, Jacquemet G. J Cell Sci jcs.265278 10.1242/jcs.265278