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MYO10_MD_pcDNA3.1(+)-N-eGFP
(Plasmid #245886)

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This material is available to academics and nonprofits only.
Item Catalog # Description Quantity Price (USD)
Plasmid 245886 Standard format: Plasmid sent in bacteria as agar stab 1 $94
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Backbone

  • Vector backbone
    pcDNA3.1(+)-N-eGFP
  • Vector type
    Mammalian Expression

Growth in Bacteria

  • Bacterial Resistance(s)
    Ampicillin, 100 μg/mL
  • Growth Temperature
    37°C
  • Growth Strain(s)
    DH5alpha
  • Copy number
    Unknown

Gene/Insert

  • Gene/Insert name
    MYO10 motor domain
  • Species
    H. sapiens (human)
  • Mutation
    boundaries 1-741 aa from full-length human MYO10
  • Entrez Gene
    MYO10 (a.k.a. MyoX)
  • Tag / Fusion Protein
    • eGFP

Cloning Information

Terms and Licenses

  • Academic/Nonprofit Terms
  • Industry Terms
    • Not Available to Industry

Trademarks:

  • Zeocin® is an InvivoGen trademark.

Depositor Comments

The MYO10_MD_pcDNA3.1(+)-N-eGFP construct was purchased from Genscript. Briefly, the gene fragment were synthesized using gene synthesis and cloned into pcDNA3.1(+)-N-eGFP at the BamHI/XhoI sites. Please visit https://doi.org/10.64898/2025.12.03.691809 for bioRxiv preprint.

How to cite this plasmid ( Back to top)

These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.

  • For your Materials & Methods section:

    MYO10_MD_pcDNA3.1(+)-N-eGFP was a gift from Guillaume Jacquemet (Addgene plasmid # 245886 ; http://n2t.net/addgene:245886 ; RRID:Addgene_245886)

  • For your References section:

    CCT8 associates with the MYO10 motor domain and regulates filopodia and breast cancer cell invasion. Popović A, Ojalill M, Touma C, Ball NJ, Miihkinen M, Joshi O, Dibus M, Pylvänäinen J, Ivaska J, Goult BT, Jacquemet G. J Cell Sci jcs.265278 10.1242/jcs.265278
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