mTalin1(R11)_V2078K
(Plasmid
#245889)
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PurposeBacterial expression of talin1(1974-2140) V2078K
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Depositing Lab
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Publication
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Sequence Information
Ordering
| Item | Catalog # | Description | Quantity | Price (USD) | |
|---|---|---|---|---|---|
| Plasmid | 245889 | Standard format: Plasmid sent in bacteria as agar stab | 1 | $94 * | |
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Backbone
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Vector backbonepET151 dTOPO
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Vector typeBacterial Expression
Growth in Bacteria
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Bacterial Resistance(s)Ampicillin, 100 μg/mL
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Growth Temperature37°C
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Growth Strain(s)DH5alpha
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Copy numberHigh Copy
Gene/Insert
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Gene/Insert nameTLN1
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SpeciesM. musculus (mouse)
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MutationV2078K
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Entrez GeneTln1 (a.k.a. Tln)
- Promoter T7
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Tag
/ Fusion Protein
- HisTag-V5 (N terminal on insert)
Cloning Information
- Cloning method Gene Synthesis
- 5′ sequencing primer T7F
- 3′ sequencing primer T7R
- (Common Sequencing Primers)
Resource Information
Terms and Licenses
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Academic/Nonprofit Terms
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Industry Terms
Trademarks:
- Zeocin® is an InvivoGen trademark.
Depositor Comments
Please visit https://doi.org/10.64898/2026.01.13.699108 for bioRxiv preprint.
These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.
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For your Materials & Methods section:
mTalin1(R11)_V2078K was a gift from Ben Goult (Addgene plasmid # 245889 ; http://n2t.net/addgene:245889 ; RRID:Addgene_245889) -
For your References section:
An Aggregation Prone Region (APR) in talin controls talin self-interactions to regulate integrin adhesion complex dynamics. Ball NJ, Turkki P, Guo Y, Tanvir MH, Le S, Chin JFL, Baker KB, Luo J, Brown NH, Hytönen VP, Yan J, Goult BT. bioRxiv 2026.01.13.699108 10.64898/2026.01.13.699108