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mTalin1(R11)_delta-helix1
(Plasmid #245892)

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Item Catalog # Description Quantity Price (USD)
Plasmid 245892 Standard format: Plasmid sent in bacteria as agar stab 1 $94 *
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Backbone

  • Vector backbone
    pET47b
  • Vector type
    Bacterial Expression

Growth in Bacteria

  • Bacterial Resistance(s)
    Kanamycin, 50 μg/mL
  • Growth Temperature
    37°C
  • Growth Strain(s)
    DH5alpha
  • Copy number
    High Copy

Gene/Insert

  • Gene/Insert name
    TLN1
  • Species
    M. musculus (mouse)
  • Entrez Gene
    Tln1 (a.k.a. Tln)
  • Promoter T7
  • Tag / Fusion Protein
    • HisTag-SUMO (N terminal on insert)

Cloning Information

Resource Information

Terms and Licenses

Trademarks:

  • Zeocin® is an InvivoGen trademark.

Depositor Comments

custom backbone. SUMO 4-98 (SMT3; UNIPROT: Q12306) with all lysines mutated to arginines was inserted between the HisTag and the HRV-3C site in pET47b.

Please visit https://doi.org/10.64898/2026.01.13.699108 for bioRxiv preprint.

How to cite this plasmid ( Back to top)

These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.

  • For your Materials & Methods section:

    mTalin1(R11)_delta-helix1 was a gift from Ben Goult (Addgene plasmid # 245892 ; http://n2t.net/addgene:245892 ; RRID:Addgene_245892)

  • For your References section:

    An Aggregation Prone Region (APR) in talin controls talin self-interactions to regulate integrin adhesion complex dynamics. Ball NJ, Turkki P, Guo Y, Tanvir MH, Le S, Chin JFL, Baker KB, Luo J, Brown NH, Hytönen VP, Yan J, Goult BT. bioRxiv 2026.01.13.699108 10.64898/2026.01.13.699108
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