pRSFDUET-ssDsbA-mCherry2_OmpX-mNeongreen
(Plasmid #245928)

• Purpose: Co-expression of ssDsbA-mCherry2 and OmpX-mNeongreen fusions in E. coli cells
• Depositing Lab: Dan Mulvihill
• Publication: VNp induced Extracellular Vesicles are distinct from natural E. coli OMVs and have properties that enhance their application for recombinant protein production. (unpublished)

Backbone

• Vector backbone: pRSFDUET-1
• Total vector size (bp): 6022
• Vector type: Bacterial Expression

Growth in Bacteria

• Bacterial Resistance(s): Kanamycin, 50 μg/mL
• Growth Temperature: 37°C
• Growth Strain(s): DH5alpha
• Copy number: High Copy

Gene/Insert 1

• Gene/Insert name: ssDsbA-mCherry2
• Species: Synthetic
• Insert Size (bp): 843
• Promoter: T7
• Tags / Fusion Proteins:
  • His6 (C terminal on insert)
  • mCherry2 (C terminal on insert)

Cloning Information for Gene/Insert 1

• Cloning method: Restriction Enzyme
• 5′ cloning site: NcoI (destroyed during cloning)
• 3′ cloning site: NotI (not destroyed)
• 5′ sequencing primer: ACYCDuetUP1

Gene/Insert 2

• Gene/Insert name: OmpX-mNeongreen
• Species: Synthetic
• Insert Size (bp): 1434
• Entrez Gene: ompX (a.k.a. b0814, ECK0803, ompP, ybiG)
• Promoter: T7
• Tag / Fusion Protein:
  • mNeongreen (C terminal on insert)

Cloning Information for Gene/Insert 2

• Cloning method: Restriction Enzyme
• 5′ cloning site: NdeI (destroyed during cloning)
• 3′ cloning site: XhoI (not destroyed)
• 5′ sequencing primer: DuetUP2 Primer
• 3′ sequencing primer: T7 Terminator Primer

Resource Information

• A portion of this plasmid was derived from a plasmid made by: Yes

Terms and Licenses

• Academic/Nonprofit Terms:
  • UBMTA
  • mNeonGreen Label License
• Industry Terms:
  • Not Available to Industry