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pRSFDUET-VNp6-mCherry2-StefinA
(Plasmid #245930)

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This material is available to academics and nonprofits only.
Item Catalog # Description Quantity Price (USD)
Plasmid 245930 Standard format: Plasmid sent in bacteria as agar stab 1 $94
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Backbone

  • Vector backbone
    pRSFDUET-1
  • Backbone manufacturer
    novagen
  • Total vector size (bp) 4960
  • Vector type
    Bacterial Expression

Growth in Bacteria

  • Bacterial Resistance(s)
    Kanamycin, 50 μg/mL
  • Growth Temperature
    37°C
  • Growth Strain(s)
    DH5alpha
  • Copy number
    High Copy

Gene/Insert

  • Gene/Insert name
    VNp6-mCherry2-StefinA
  • Species
    Synthetic
  • GenBank ID
    P01040
  • Entrez Gene
    CSTA (a.k.a. AREI, PSS4, STF1, STFA)
  • Promoter T7
  • Tag / Fusion Protein
    • His6 (C terminal on insert)

Cloning Information

  • Cloning method Restriction Enzyme
  • 5′ cloning site NdeI (not destroyed)
  • 3′ cloning site XhoI (not destroyed)
  • 5′ sequencing primer DuetUP2 Primer
  • 3′ sequencing primer T7 Terminator Primer
  • (Common Sequencing Primers)

Resource Information

  • A portion of this plasmid was derived from a plasmid made by
    Yes

Terms and Licenses

  • Academic/Nonprofit Terms
  • Industry Terms
    • Not Available to Industry

Trademarks:

  • Zeocin® is an InvivoGen trademark.
How to cite this plasmid ( Back to top)

These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.

  • For your Materials & Methods section:

    pRSFDUET-VNp6-mCherry2-StefinA was a gift from Dan Mulvihill (Addgene plasmid # 245930 ; http://n2t.net/addgene:245930 ; RRID:Addgene_245930)
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