Skip to main content
Addgene
Open Menu

pRSFDUET-ssDsbA-mNeongreen
(Plasmid #245932)

Print

Ordering

This material is available to academics and nonprofits only.
Item Catalog # Description Quantity Price (USD)
Plasmid 245932 Standard format: Plasmid sent in bacteria as agar stab 1 $94
Add to Cart

Backbone

  • Vector backbone
    pRSFDUET-1
  • Backbone manufacturer
    novagen
  • Vector type
    Bacterial Expression

Growth in Bacteria

  • Bacterial Resistance(s)
    Kanamycin, 50 μg/mL
  • Growth Temperature
    37°C
  • Growth Strain(s)
    DH5alpha
  • Copy number
    Low Copy

Gene/Insert

  • Gene/Insert name
    Periplasmic targeting sequence ssDsbA fused to mNeongreen
  • Species
    Synthetic
  • Insert Size (bp)
    834
  • Promoter T7
  • Tag / Fusion Protein
    • His6 (C terminal on insert)

Cloning Information

  • Cloning method Restriction Enzyme
  • 5′ cloning site Nde1 (not destroyed)
  • 3′ cloning site Xho1 (not destroyed)
  • 5′ sequencing primer DuetUP2 Primer
  • 3′ sequencing primer T7 terminator
  • (Common Sequencing Primers)

Resource Information

  • A portion of this plasmid was derived from a plasmid made by
    Yes

Terms and Licenses

Trademarks:

  • Zeocin® is an InvivoGen trademark.
How to cite this plasmid ( Back to top)

These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.

  • For your Materials & Methods section:

    pRSFDUET-ssDsbA-mNeongreen was a gift from Dan Mulvihill (Addgene plasmid # 245932 ; http://n2t.net/addgene:245932 ; RRID:Addgene_245932)
Related items: