huCof C39S-mRFP
(Plasmid
#245967)
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PurposeExpression of cof with one of 4 cys mutated to ser. Incorporates into cofilactin rods
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Depositing Lab
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Depositing OrganizationColorado State University, Fort Collins
Located in the United States of America -
Sequence Information
Ordering
| Item | Catalog # | Description | Quantity | Price (USD) | |
|---|---|---|---|---|---|
| Plasmid | 245967 | Standard format: Plasmid sent in bacteria as agar stab | 1 | $94 * | |
| DNA | 245967-D50 | 50 μg of DNA in Tris buffer | $495 * | ||
| DNA | 245967-D100 | 100 μg of DNA in Tris buffer | $585 * | ||
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Backbone
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Vector backbonepShuttle CMV
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Backbone manufacturerGift from Bert Vogelstein in 1999
- Backbone size w/o insert (bp) 7470
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Vector typeMammalian Expression
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Selectable markersNeomycin (select with G418)
Growth in Bacteria
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Bacterial Resistance(s)Kanamycin, 50 μg/mL
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Growth Temperature37°C
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Growth Strain(s)DH5alpha
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Copy numberHigh Copy
Gene/Insert
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Gene/Insert namecofilin 1 (cfl1)
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SpeciesH. sapiens (human)
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MutationC39S
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Entrez GeneCFL1 (a.k.a. CFL, HEL-S-15, cofilin)
- Promoter CMV
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Tag
/ Fusion Protein
- mRFP (C terminal on insert)
Cloning Information
- Cloning method Restriction Enzyme
- 5′ cloning site Unspecified (unknown if destroyed)
Terms and Licenses
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Academic/Nonprofit Terms
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Industry Terms
Trademarks:
- Zeocin® is an InvivoGen trademark.
DNA (Catalog # 245967-D50)
Purpose
Ready-to-use, high-purity DNA prep. DNA aliquots are suitable for use in mammalian cell transfections and other molecular biology applications.
Delivery
- Amount 50 μg
- Concentration 1 μg/μL
- Pricing $495 USD
- Storage 4 ℃ (short-term) or -20 ℃ (long-term)
Terms and Licenses
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Academic/Nonprofit Terms
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Industry Terms
Quality Control
Every DNA prep is verified by next-generation sequencing, confirming plasmid identity, sequence integrity, and absence of DNA contaminants. Concentration is confirmed to be within ±2.5% of the stated concentration.
Our production process has been validated to consistently yield endotoxin levels below 1.0 EU/μg. Individual lots are not tested for endotoxin.
DNA (Catalog # 245967-D100)
Purpose
Ready-to-use, high-purity DNA prep. DNA aliquots are suitable for use in mammalian cell transfections and other molecular biology applications.
Delivery
- Amount 100 μg
- Concentration 1 μg/μL
- Pricing $585 USD
- Storage 4 ℃ (short-term) or -20 ℃ (long-term)
Terms and Licenses
-
Academic/Nonprofit Terms
-
Industry Terms
Quality Control
Every DNA prep is verified by next-generation sequencing, confirming plasmid identity, sequence integrity, and absence of DNA contaminants. Concentration is confirmed to be within ±2.5% of the stated concentration.
Our production process has been validated to consistently yield endotoxin levels below 1.0 EU/μg. Individual lots are not tested for endotoxin.
These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.
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For your Materials & Methods section:
huCof C39S-mRFP was a gift from James Bamburg (Addgene plasmid # 245967 ; http://n2t.net/addgene:245967 ; RRID:Addgene_245967) -
For your References section:
Incorporation of cofilin into rods depends on disulfide intermolecular bonds: implications for actin regulation and neurodegenerative disease. Bernstein BW, Shaw AE, Minamide LS, Pak CW, Bamburg JR. J Neurosci. 2012 May 9;32(19):6670-81. doi: 10.1523/JNEUROSCI.6020-11.2012. 10.1523/JNEUROSCI.6020-11.2012 PubMed 22573689
