pJD486
(Plasmid #246005)

• Purpose: (Empty Backbone) A plasmid for Massively Parallel Reporter Assays of 5'UTR function. It includes a CMV promoter, ITRs for AAV packaging, cloning sites. This is derived from pJD460 (Addgene #246006) by adding reporter.
• Depositing Lab: Joseph Dougherty
• Publication: Approaches for Identification of 5′UTR Mutations Impacting Translation and Protein Production from Neurodevelopmental Disorder Genes (unpublished)

Backbone

• Vector backbone: pJD460
• Modifications to backbone: Exactly as 460, but with an example 5'UTR cloned in and an example barcode cloned in.
• Vector type: Mammalian Expression, AAV, Cre/Lox
• Promoter: cmv
• Tag / Fusion Protein:
  • N-terminal Myc Tag on a TdTomato (which has an intron from beta globin) (N terminal on insert)

Growth in Bacteria

• Bacterial Resistance(s): Ampicillin, 100 μg/mL
• Growth Temperature: 37°C
• Growth Strain(s): NEB Stable
• Copy number: High Copy

Cloning Information

• Cloning method: Restriction Enzyme
• 5′ sequencing primer: CMV fwd primer

Terms and Licenses

• Academic/Nonprofit Terms:
  • UBMTA
• Industry Terms:
  • Not Available to Industry

Depositor Comments

Please note that the depositor's annotated reference file has minor differences compared to the Addgene verified sequence. Please refer to the the Addgene sequence for the most accurate result.