pJD460
(Plasmid
#246006)
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Purpose(Empty Backbone) A plasmid for Massively Parrallel Reporter Assays of 5'UTR function. It includes a CMV promoter, ITRs for AAV packaging, cloning sites. This does not include elements or reporters.
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Depositing Lab
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Sequence Information
Ordering
This material is available to academics and nonprofits only.
| Item | Catalog # | Description | Quantity | Price (USD) | |
|---|---|---|---|---|---|
| Plasmid | 246006 | Standard format: Plasmid sent in bacteria as agar stab | 1 | $94 | |
Backbone
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Vector backboneNA
- Backbone size (bp) 6020
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Vector typeMammalian Expression, AAV, Cre/Lox
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Tag
/ Fusion Protein
- N-terminal Myc Tag on a TdTomato (which has an intron from beta globin)
Growth in Bacteria
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Bacterial Resistance(s)Ampicillin, 100 μg/mL
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Growth Temperature37°C
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Growth Strain(s)NEB Stable
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Copy numberHigh Copy
Cloning Information
- 5′ sequencing primer CMV fwd primer
- (Common Sequencing Primers)
Resource Information
Terms and Licenses
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Academic/Nonprofit Terms
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Industry Terms
- Not Available to Industry
Trademarks:
- Zeocin® is an InvivoGen trademark.
Depositor Comments
Please note that the depositor's annotated reference file has minor differences compared to the Addgene verified sequence. Please refer to the the Addgene sequence for the most accurate result.
How to cite this plasmid
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These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.
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For your Materials & Methods section:
pJD460 was a gift from Joseph Dougherty (Addgene plasmid # 246006 ; http://n2t.net/addgene:246006 ; RRID:Addgene_246006)
